Interleukin 7 and sCD23 Synergize in the Induction of Human T Cell Activation in HIV-1-Infected Subjects
| Autor: | Claudio Carini, Mirella Guerriero, Candida Fratazzi |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Cytotoxicity Immunologic Male T-Lymphocytes medicine.medical_treatment T cell Immunology HIV Infections CD8-Positive T-Lymphocytes Biology Lymphocyte Activation Interferon-gamma Immune system Antigen medicine Humans Immunology and Allergy Cytotoxic T cell Interferon gamma Receptors IgE Interleukin-7 Gene Products env Interleukin Drug Synergism General Medicine T lymphocyte Interleukin-12 Cytokine medicine.anatomical_structure Solubility Influenza A virus HIV-1 Interleukin-2 medicine.drug |
| Zdroj: | International Archives of Allergy and Immunology. 114:120-129 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000237656 |
| Popis: | The role played by CD23 in retroviral infections is still unclear. The synergistic effects of interleukin 7 (IL-7) and sCD23 on T cell proliferation and the generation of HIV-1-specific cytotoxic T lymphocytes (CTL) in mitogen- and antigen-stimulated systems were examined. Addition of IL-7 and sCD23 at the onset of culture resulted in a marked augmentation of T cell proliferation and cytotoxic activity. Studies of CTL development in purified mitogen CD8+ T cells demonstrated that IL-7 and sCD23 could act directly on the CD8+ lymphocyte subset to augment cytotoxicity. The data demonstrate that IL-7 and sCD23 synergistically augmented CTL activity independently of IL-2 and IL-12. We analyzed the effects on IFN-gamma production by CD8+ T cells and found that IL-7 alone did not induce detectable levels of IFN-gamma production, but together with sCD23, it synergistically enhanced the production of IFN-gamma. We also found that IFN-gamma appeared not to be required for the enhanced CD8+ CTL activity because rabbit anti-IFN-gamma antibody did not block the synergistic effects of IL-7 and sCD23. These results indicate that IL-7 and sCD23 can exert major upregulatory effects on human CTL development and suggest that these effects are both proliferative and differentiative. |
| Databáze: | OpenAIRE |
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