Optimization of Plant Extract Purification Procedure for Rapid Screening Analysis of Sixteen Phenolics by Liquid Chromatography
| Autor: | Ildikó Matušíková, Peter Nemeček, Petra Ranušová |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
plant extracts
phenolics Filtration and Separation 01 natural sciences Protocatechuic acid Analytical Chemistry Ferulic acid lcsh:Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Chlorogenic acid Caffeic acid Vanillic acid solid-phase extraction Gallic acid Gentisic acid high-performance liquid chromatography metabolites Chromatography Phenylpropanoid 010401 analytical chemistry food and beverages 04 agricultural and veterinary sciences 040401 food science lcsh:QC1-999 0104 chemical sciences chemistry lcsh:QD1-999 lcsh:Physics |
| Zdroj: | Separations, Vol 8, Iss 13, p 13 (2021) Separations Volume 8 Issue 2 |
| ISSN: | 2297-8739 |
| Popis: | A solid-phase extraction (SPE) procedure was developed for simultaneous monitoring of sixteen different phenolics of various polarity, quantified by high-performance liquid chromatography (HPLC). The procedure allowed screening the accumulation of intermediates in different metabolic pathways that play a crucial role in plant physiology and/or are beneficial for human health. Metabolites mostly involved in phenylpropanoid, shikimate, and polyketide pathways comprise chlorogenic acid, gentisic acid, vanillic acid, caffeic acid, protocatechuic acid, ferulic acid, rutin, quercetin, epicatechin, gallic acid, sinapic acid, p-coumaric acid, o-coumaric acid, vanillin two rarely quantified metabolites, 2,5-dimethoxybenzoic acid and 4-methoxycinnamic acid, were included as well. The procedure offered low cost, good overall efficiency, and applicability in laboratories with standard laboratory equipment. SPE recoveries were up to 99.8% at various concentration levels. The method allowed for routine analysis of compounds with a wide range of polarity within a single run, while its applicability was demonstrated for various model plant species (tobacco, wheat, and soybean), as well as different tissue types (shoots and roots). |
| Databáze: | OpenAIRE |
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