A single immunization with a dry powder anthrax vaccine protects rabbits against lethal aerosol challenge
| Autor: | A.M. Cheff, S.J. Warwood, J.P. Stenz, Charles Richardson, S.D. Klas, C.L. Olds, Marlene S. Williams, C.R. Petrie, M. Hinchcliffe, S. Wimer |
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| Rok vydání: | 2008 |
| Předmět: |
Chemistry
Pharmaceutical Enzyme-Linked Immunosorbent Assay Anthrax Vaccines Biology complex mixtures Median lethal dose Article Immunoglobulin G Microbiology Anthrax Adjuvants Immunologic Neutralization Tests Animals Neutralizing antibody Administration Intranasal Bacterial Capsules Aerosols Antigens Bacterial Vaccines Synthetic Anthrax vaccines General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health biology.organism_classification Antibodies Bacterial Virology Bacillus anthracis Solutions enzymes and coenzymes (carbohydrates) Titer Infectious Diseases Immunization Vaccines Subunit biology.protein Alum Compounds Molecular Medicine Female Indicators and Reagents Nasal administration Rabbits Powders |
| Zdroj: | Vaccine. 26:5494-5502 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2008.07.062 |
| Popis: | Here we confirm that intranasal (IN) dry powder anthrax vaccine formulations are able to protect rabbits against aerosol challenge 9 weeks after a single immunization. The optimum dose of rPA in our dry powder anthrax vaccine formulation in rabbits was experimentally determined to be 150 μg and therefore was chosen as the target dose for all subsequent experiments. Rabbits received a single dose of either 150 μg rPA, 150 μg rPA + 150 μg of a conjugated 10-mer peptide representing the Bacillus anthracis capsule (conj), or 150 μg of conj alone. All dry powder formulations contained MPL and chitosan (ChiSys ® ). Significant anti-rPA titers and anthrax lethal toxin neutralizing antibody (TNA) levels were seen with both rPA containing vaccines, although rPA-specific IgG and TNA levels were reduced in rabbits immunized with rPA plus conj. Nine weeks after immunization, rabbits were exposed to a mean aerosol challenge dose of 278 LD 50 of Ames spores. Groups immunized with rPA or with rPA + conj had significant increases in survivor proportions compared to the negative control group by Logrank test ( p = 0.0001 and 0.003, respectively), and survival was not statistically different for the rPA and rPA + conj immunized groups ( p = 0.63). These data demonstrate that a single immunization with our dry powder anthrax vaccine can protect against a lethal aerosol spore challenge 9 weeks later. |
| Databáze: | OpenAIRE |
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