Targeted overexpression of tumor necrosis factor-α increases cyclin-dependent kinase 5 activity and TRPV1-dependent Ca2+ influx in trigeminal neurons
| Autor: | Ricardo Piña, Pablo Rozas, Ashok B. Kulkarni, Rodolfo Madrid, Christian Gonzalez-Billault, María Pertusa, Andrew Cho, Pablo Lazcano, Elias Utreras, Anita Terse |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetically modified mouse Orofacial pain Sensory Receptor Cells TRPV Cation Channels Cre recombinase Mice Transgenic CCL2 Article Proinflammatory cytokine Mice 03 medical and health sciences Trigeminal ganglion 0302 clinical medicine medicine Animals Chemokine CCL2 Tumor Necrosis Factor-alpha Chemistry Macrophages Cyclin-Dependent Kinase 5 030104 developmental biology Anesthesiology and Pain Medicine Trigeminal Ganglion nervous system Neurology Cancer research Calcium Tumor necrosis factor alpha Neurology (clinical) medicine.symptom Cell activation 030217 neurology & neurosurgery |
| Zdroj: | Pain Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 0304-3959 |
| Popis: | We reported earlier that TNF-α, a proinflammatory cytokine implicated in many inflammatory disorders causing orofacial pain, increases the activity of Cdk5, a key kinase involved in brain development and function and recently found to be involved in pain signaling. To investigate a potential mechanism underlying inflammatory pain in trigeminal ganglia (TGs), we engineered a transgenic mouse model (TNF) that can conditionally overexpresses TNF-α upon genomic recombination by Cre recombinase. TNF mice were bred with Nav1.8-Cre mouse line that expresses the Cre recombinase in sensory neurons to obtain TNF-α:Nav1.8-Cre (TNF-α cTg) mice. Although TNF-α cTg mice appeared normal without any gross phenotype, they displayed a significant increase in TNF-α levels after activation of NFκB signaling in the TG. IL-6 and MCP-1 levels were also increased along with intense immunostaining for Iba1 and GFAP in TG, indicating the presence of infiltrating macrophages and the activation of satellite glial cells. TNF-α cTg mice displayed increased trigeminal Cdk5 activity, and this increase was associated with elevated levels of phospho-T407-TRPV1 and capsaicin-evocated Ca influx in cultured trigeminal neurons. Remarkably, this effect was prevented by roscovitine, an inhibitor of Cdk5, which suggests that TNF-α overexpression induced sensitization of the TRPV1 channel. Furthermore, TNF-α cTg mice displayed more aversive behavior to noxious thermal stimulation (45°C) of the face in an operant pain assessment device as compared with control mice. In summary, TNF-α overexpression in the sensory neurons of TNF-α cTg mice results in inflammatory sensitization and increased Cdk5 activity; therefore, this mouse model would be valuable for investigating the mechanism of TNF-α involved in orofacial pain. |
| Databáze: | OpenAIRE |
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