Screening of the potentially active compounds from Polygonatum sibiricum using RAW264.7 cellular membranes coated magnetic beads fishing followed by HPLC analysis
| Autor: | Cheng Tang, Ya-Ming Yu, Qing-Ling Qi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Polygonatum sibiricum
Clinical Biochemistry Phytochemicals 030226 pharmacology & pharmacy 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry 03 medical and health sciences Mice 0302 clinical medicine Drug Discovery Animals Molecular Biology Chromatography High Pressure Liquid Pharmacology Hplc analysis Chromatography Chemistry Plant Extracts 010401 analytical chemistry Cell Membrane Polygonatum Macrophage cell Biological activity General Medicine 0104 chemical sciences Membrane RAW 264.7 Cells Active compound Magnetic bead Magnets |
| Zdroj: | Biomedical chromatography : BMCREFERENCES. 34(2) |
| ISSN: | 1099-0801 |
| Popis: | Target biomolecule-immobilized magnetic beads could be used as a powerful tool for screening active compounds present in natural products. Low damage rates of the target proteins, associated with the availability of diverse automated online approaches for analysis, make it a valuable tool for affinity studies. RAW264.7 cells (a kind of murine macrophage cell line) were used in this study. These cellular membranes were immobilized onto the surface of MBs and were used for screening the active compounds of Polygonatum sibiricum. Combining this technique with HPLC led to the identification of an active compound and its biological activity was confirmed. This is the first report establishing the use of RAW264.7 cellular membrane-coated magnetic bead fishing followed by HPLC analysis for screening active compounds from natural products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text