Evaluation of Liver Regeneration Using the -[1-13C]Methionine Breath Test
| Autor: | Tomohisa Kohno, Yasuo Takahashi, Koichi Ishikawa, Shigeru Suzuki, Tadashi Kohno, Toshihito Nagata, Yukimoto Ishii, Shigetomi Iwai, Satoshi Asai |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Pathology Time Factors medicine.medical_treatment Rat model Femoral vein Body weight Liver weight Gastroenterology chemistry.chemical_compound Methionine Proliferating Cell Nuclear Antigen Internal medicine medicine Animals Hepatectomy Rats Wistar Breath test Carbon Isotopes medicine.diagnostic_test business.industry Reproducibility of Results DNA Carbon Dioxide Liver regeneration Liver Regeneration Rats Breath Tests Liver chemistry Models Animal Surgery business |
| Zdroj: | Journal of Surgical Research. 95:195-199 |
| ISSN: | 0022-4804 |
| DOI: | 10.1006/jsre.2000.6012 |
| Popis: | Background. We examined the relationship between changes in the liver weight/body weight percentage, amount of hepatic tissue total DNA, and the results of the [1- 13 C]methionine ( 13 Cmet) breath test during hepatic regeneration in a rat model of 70% hepatectomy, to assess their usefulness for evaluating hepatic regeneration. Materials and methods. Male Wistar rats (230–290 g) were subjected to 70% hepatectomy under anesthesia with Nembutal. One, 2, 3, 7, and 14 days postoperatively, 40 mg/kg 13 Cmet was intravenously injected into the femoral vein, and the increase in exhaled 13 CO 2 (Δ 13 CO 2 ) was measured for 15 min. Simple laparotomy was performed in control rats. Following the breath test, the regenerated liver was removed and weighed. The amount of DNA was determined. Results. The correlation coefficients ( r ) between liver weight/body weight (LW/BW) and results of the 13 Cmet breath test, and between DNA and results of the 13 Cmet breath test were 0.892 and 0.800, respectively. Conclusions. The 13 Cmet breath test is considered to be very useful for assessing liver regeneration, and total 13 CO 2 output over 15 min in the 13 Cmet breath test graph seems to be an effective indicator for evaluating liver regeneration. |
| Databáze: | OpenAIRE |
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