| Popis: |
The emergence of resistance to previously established antimalarial agents, have created the need for a continued effort geared towards the discovery of newer agents. The present study involves an in vivo evaluation of the potential effect of selected antioxidant micronutrient combination in the therapeutics of malaria. In this study, rodent malaria model; Plasmodium berghei NK-65 strain (chloroquine sensitive) and chloroquine resistant ANKA strain was used. In the first stage of the experiment, a 4 day curative synergistic test was conducted using 45 mice of either sex weighing 20.05±0.02 g which were inoculated intraperitoneally with 1 x 107 million Plasmodium berghei infected erythrocyte and were administered with varying combination of selected micronutrients for 4 days, 72 hours post inoculation. The second stage involved the use of 45 mice of either sex; curative synergistic study using chloroquine resistant ANKA strain was conducted. Similarly, varying combination of micronutrients with standard antimalarial agents was administered for 4 days. Synergistic schizonticidal activity was most marked in the vitamin A + E combination group (94.52%) when compared with any other micronutrient combination group after 4 days treatment of established infection. This was closely followed by the vitamin A + selenium combination group (94.43%). The mean difference in parasitemic levels was significant between groups (F = 2.59; P < 0.05). Conclusively, antioxidant micronutrient combination has potential benefit of being used as adjuvant in malaria therapeutics. |
