Encapsulation of Adenovirus BMP2-Transduced Cells with PEGDA Hydrogels Allows Bone Formation in the Presence of Immune Response
| Autor: | Edward V. Strecker, Alan R. Davis, Pedro Alvarez-Urena, Eleanor L. Davis, Gabrielle Henslee, Corinne Sonnet, Elizabeth A. Olmsted-Davis, Jennifer L. West, Maude L. Cuchiara, Laura J. Linscheid, Zbigniew Gugala |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetic enhancement Biomedical Engineering Bone Morphogenetic Protein 2 Bioengineering Mice SCID medicine.disease_cause Biochemistry Bone morphogenetic protein 2 Adenoviridae Polyethylene Glycols Biomaterials Cell therapy Mice 03 medical and health sciences Immune system Mice Inbred NOD Transduction Genetic Immunity medicine Animals biology Chemistry Hydrogels Original Articles Cells Immobilized Fibroblasts Cell biology 030104 developmental biology Immunology Self-healing hydrogels biology.protein Antibody |
| Zdroj: | Tissue Engineering Part A. 23:177-184 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2016.0277 |
| Popis: | Gene therapy approaches have been difficult to implement due to pre-existing immunity against the virus used for delivery. To circumvent this problem, a cell-based approach was developed that avoided the use of free virus within the animal. However, even cells transduced in vitro with E1- to E3-deleted adenovirus encoding bone morphogenetic protein 2 (AdBMP2) resulted in the production of virus-neutralizing antibodies in mice. Furthermore, when mice received an intramuscular injection of nonencoding adenovirus (AdEmpty)-transduced cells, AdBMP2-transduced cells were unable to launch bone formation when an intramuscular injection of these BMP2-producing cells was delivered 1 week later. This phenomenon was not observed in NOD/SCID mice, and could be overcome in C57BL/6 mice by encapsulating the adenovirus-transduced cells in a nondegradable hydrogel poly(ethylene glycol) diacrylate (PEGDA). Data collectively suggest that PEGDA hydrogel encapsulation of AdBMP2-transduced cells prevents pre-existing immunity from suppressing BMP2-induced bone formation. |
| Databáze: | OpenAIRE |
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