Phenolic profile and effects of acetone fractions obtained from the inflorescences of Calluna vulgaris (L.) Hull on vaginal pathogenic and non-pathogenic bacteria
| Autor: | Paulo F. Santos, Maria Inês Dias, Filipa Mandim, Sandrina A. Heleno, Isabel C.F.R. Ferreira, Lillian Barros, Tânia C.S.P. Pires, Maria José Alves |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microorganism Flowers Microbial Sensitivity Tests medicine.disease_cause 03 medical and health sciences Column chromatography Phenols Lactobacillus medicine Humans Food science 2. Zero hunger 030109 nutrition & dietetics biology Bacteria Chemistry Plant Extracts Pathogenic bacteria General Medicine biology.organism_classification 3. Good health 030104 developmental biology Vagina Female Morganella morganii Antibacterial activity Methicillin Susceptible Staphylococcus Aureus Food Science Calluna |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 2042-650X |
| Popis: | The phenolic profile and the antibacterial activity against pathogenic commensal vaginal bacteria exhibited by different fractions of the acetone extract of heather was assessed. The acetone extract of Calluna vulgaris (L.) Hull was fractionated by silica gel column chromatography through an eluent system of increasing polarity, obtaining 10 different fractions (Fr 1 to Fr 10). The phenolic profile was analyzed by HPLCDAD- ESI/MS. Type B (epi)catechin dimers, (-)-epicatechin and (+)-catechin were the main phenolic compounds present in the fractions. The antibacterial activity was also analyzed against pathogenic bacteria and the effect in the beneficial microflora was also accessed. Some of the obtained fractions revealed the capacity to inhibit pathogenic microorganisms without affecting the beneficial microflora, especially Fr 7 and Fr 8. For instance, Neisseria gonorrhoeae was inhibited by both of the fractions, while Fr 7 was more active against Klebsiella pneumoniae and Morganella morganii, and Fr 8 against methicillin resistance Staphylococcus aureus (MRSA) and methicillin susceptible Staphylococcus aureus (MSSA), without affecting Lactobacillus strains. This study corroborates the therapeutic use of this matrix in traditional medicine. The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programmer PT2020 for financial support to CIMO (UID/AGR/00690/2019), CQ-VR (UID/QUI/00616/2013), and to FEDER-Interreg España-Portugal program for financial support through the project 0377_Iberphenol_6_E. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for Sandrina A. Heleno (SFRH/BPD/101413/2014) grant and L. Barros contract. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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