Elastic modulus of suspended purple membrane measured by atomic force microscopy
| Autor: | Keiichi Torimitsu, Kazuaki Furukawa, John F. Ryan, Koji Sumitomo, Nahoko Kasai, Ari M. Siitonen, Chandra S. Ramanujan, Youichi Shinozaki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
biology
Chemistry technology industry and agriculture Analytical chemistry General Physics and Astronomy Bacteriorhodopsin Biological membrane Surfaces and Interfaces General Chemistry Substrate (electronics) Condensed Matter Physics Surfaces Coatings and Films Crystal Scanning probe microscopy Membrane biology.protein Thin film Elastic modulus |
| Zdroj: | APPLIED SURFACE SCIENCE. 254(23) |
| ISSN: | 0169-4332 |
| Popis: | We have probed the mechanical properties of purple membrane (PM) in a physiological environment using the atomic force microscope (AFM). By suspending PM over nano-trenches, the elastic properties of PM can be evaluated free from the interaction with the substrate. Force-displacement curves were obtained on the suspended membrane and the data was compared to that of a simple model of a thin film over a trench. By fitting the data to the model, the elastic modulus of PM was estimated to be 8 MPa. When the membrane is repeatedly indented, we observed a change in the force-distance data consistent with damage to the two-dimensional crystal of PM. In this paper we demonstrate that the AFM allows us to evaluate the mechanics of biological membranes in their native conditions. © 2008 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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