Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1
Autor: | Scott S. Strugnell, Ivan R. Nabi, Liliana D. Kojic, Trevor Scudamore, Jacky G. Goetz, Bharat H. Joshi, Patrick Lajoie |
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Rok vydání: | 2008 |
Předmět: |
Macromolecular Substances
Galectin 3 Caveolin 1 Nerve Tissue Proteins Biology N-Acetylglucosaminyltransferases Article Cell membrane Focal adhesion Mice 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor Cell Adhesion medicine Animals Humans Neoplasm Invasiveness Amino Acid Sequence Phosphorylation Tyrosine Cell adhesion Research Articles Cytoskeleton 030304 developmental biology Galectin Focal Adhesions 0303 health sciences Cell Membrane Cell Biology 3. Good health Cell biology Protein Transport Crosstalk (biology) medicine.anatomical_structure Focal Adhesion Protein-Tyrosine Kinases 030220 oncology & carcinogenesis Protein Binding |
Zdroj: | The Journal of Cell Biology |
ISSN: | 1540-8140 0021-9525 |
Popis: | Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesion kinase (FAK) within FAs, and thereby promotes FA disassembly and turnover. Expression of the Mgat5/galectin lattice alone induces FAs and cell spreading. However, FAK stabilization in FAs also requires expression of pY14Cav1. In cells lacking the Mgat5/galectin lattice, pY14Cav1 is not sufficient to promote FAK stabilization, FA disassembly, and turnover. In human MDA-435 cancer cells, Cav1 expression, but not mutant Y14FCav1, stabilizes FAK exchange and stimulates de novo FA formation in protrusive cellular regions. Thus, transmembrane crosstalk between the galectin lattice and pY14Cav1 promotes FA turnover by stabilizing FAK within FAs defining previously unknown, interdependent roles for galectin-3 and pY14Cav1 in tumor cell migration. |
Databáze: | OpenAIRE |
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