Association studies using random and 'candidate' microsatellite loci in two infectious goat diseases

Autor: Ursula Sattler, Christophe Chartier, Claude Gaillard, Marie-Louise Glowatzki, Dominique Martinez, Jean-Charles Maillard, Gabriela Obexer-Ruff, Nasikat Saitbekova
Jazyk: angličtina
Rok vydání: 2003
Předmět:
0106 biological sciences
01 natural sciences
microsatellites
MHC Class II Gene
Genetics(clinical)
Chèvre
lcsh:SF1-1100
Genetics
0303 health sciences
Goat Diseases
biology
Trichostrongylosis
General Medicine
Microsatellite
Cytokines
Disease Susceptibility
Heartwater Disease
Génétique moléculaire
goats
lcsh:QH426-470
Genes
MHC Class II
Locus (genetics)
Bovidae
010603 evolutionary biology
03 medical and health sciences
Animals
Allele
Gene
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Genetic association
Research
disease association
L10 - Génétique et amélioration des animaux
Résistance aux maladies
biology.organism_classification
lcsh:Genetics
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
Maladie infectieuse
Animal Science and Zoology
lcsh:Animal culture
Microsatellite Repeats
Zdroj: Genetics, Selection, Evolution : GSE
Genetics Selection Evolution
Genetics Selection Evolution, BioMed Central, 2003, 35 (Suppl. 1), pp.S113-S119. ⟨10.1051/gse:2003020⟩
Genetics Selection Evolution, Vol 35, Iss Suppl 1, Pp S113-S119 (2003)
ISSN: 1297-9686
0999-193X
Popis: We established a set of 30 microsatellites of Bovidae origin for use in a biodiversity study in Swiss and Creole goats. Additional microsatellites located within or next to "candidate" genes of interest, such as cytokine genes (IL4, INF-gamma) and MHC class II genes (DRB, DYA) were tested in the caprine species in order to detect possible associations with two infectious caprine diseases. Microsatellite analysis was undertaken using automated sequencers (ABI373 & 3100). In the first study, a total of 82 unrelated Creole goats, 37 resistant and 45 susceptible to Heartwater disease (Cowdriosis) were analysed. In this study, the two microsatellite loci DRBP1 (MHCII) and BOBT24 (IL4) were positively associated with disease susceptibility, demonstrating a corrected P-value of 0.002 and 0.005, respectively. In a second investigation, we tested 36 goats, naturally infected with the nematode parasite Trichostrongylus colubriformis. These animals were divided into a "low" and "high" excreting group on the basis of two independently recorded fecal egg counts. For this nematode resistance study, we detected a significant association of one of the alleles of the microsatellite locus SPS113 with "low" excretion (resistance). The MHC class II locus DYA (P19), was weakly associated with susceptibility in both diseases (Pc = 0.05). In future experiments, we will extend the sample size in order to verify the described associations.
Databáze: OpenAIRE
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