LC-HRMS Metabolomics for Untargeted Diagnostic Screening in Clinical Laboratories: A Feasibility Study
| Autor: | Pierre-Edouard Sottas, Rayane Mohamed, Bertrand Rochat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism lcsh:QR1-502 diagnostic 01 natural sciences Biochemistry Data treatment lcsh:Microbiology Article clinical/biomedical analysis untargeted 03 medical and health sciences Metabolomics Metabolome Medicine liquid chromatography Overdiagnosis Diagnostic screening Molecular Biology forensic/toxicology Chromatography business.industry Healthy population screening 010401 analytical chemistry Forensic toxicology metabolomics Disease etiology 0104 chemical sciences 030104 developmental biology high resolution mass spectrometry business |
| Zdroj: | Metabolites Volume 8 Issue 2 Metabolites, Vol 8, Iss 2, p 39 (2018) |
| ISSN: | 2218-1989 |
| DOI: | 10.3390/metabo8020039 |
| Popis: | Today&rsquo s high-resolution mass spectrometers (HRMS) allow bioanalysts to perform untargeted/global determinations that can reveal unexpected compounds or concentrations in a patient&rsquo s sample. This could be performed for preliminary diagnosis attempts when usual diagnostic processes and targeted determinations fail. We have evaluated an untargeted diagnostic screening (UDS) procedure. UDS is a metabolome analysis that compares one sample (e.g., a patient) with control samples (a healthy population). Using liquid chromatography (LC)-HRMS full-scan analysis of human serum extracts and unsupervised data treatment, we have compared individual samples that were spiked with one xenobiotic or a higher level of one endogenous compound with control samples. After the use of different filters that drastically reduced the number of metabolites detected, the spiked compound was eventually revealed in each test sample and ranked. The proposed UDS procedure appears feasible and reliable to reveal unexpected xenobiotics (toxicology) or higher concentrations of endogenous metabolites. HRMS-based untargeted approaches could be useful as preliminary diagnostic screening when canonical processes do not reveal disease etiology nor establish a clear diagnosis and could reduce misdiagnosis. On the other hand, the risk of overdiagnosis of this approach should be reduced with mandatory biomedical interpretation of the patient&rsquo s UDS results and with confirmatory targeted and quantitative determinations. |
| Databáze: | OpenAIRE |
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