Sex differences after chronic stress in the expression of opioid-, stress- and neuroplasticity-related genes in the rat hippocampus
Autor: | Mary Jeanne Kreek, Teresa A. Milner, Sanoara Mazid, Jason D. Gray, Matthew Randesi, Yan Zhou, Shannon C. Odell, Bruce S. McEwen, J. Correa da Rosa |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Physiology medicine.drug_class Hippocampus Corticotrophin releasing factor Biology Hippocampal formation Biochemistry lcsh:RC346-429 lcsh:RC321-571 03 medical and health sciences Cellular and Molecular Neuroscience Corticotropin-releasing hormone 0302 clinical medicine Endocrinology Opioid receptor Internal medicine Gene expression medicine Drug addiction Chronic stress Original Research Article Neural plasticity Opioid peptide Molecular Biology lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry lcsh:Neurology. Diseases of the nervous system Endocrine and Autonomic Systems Dentate gyrus lcsh:QP351-495 030104 developmental biology lcsh:Neurophysiology and neuropsychology Delta opioid receptor 030217 neurology & neurosurgery |
Zdroj: | Neurobiology of Stress Neurobiology of Stress, Vol 8, Iss, Pp 33-41 (2018) |
ISSN: | 2352-2895 |
Popis: | Opioid peptides and their receptors re-organize within hippocampal neurons of female, but not male, rats following chronic immobilization stress (CIS) in a manner that promotes drug-related learning. This study was conducted to determine if there are also sex differences in gene expression in the hippocampus following CIS. Adult female and male rats were subjected to CIS (30 min/day) for 10 days. Twenty-four hours after the last stressor, the rats were euthanized, the brains were harvested and the medial (dentate gyrus/CA1) and lateral (CA2/CA3) dorsal hippocampus were isolated. Following total RNA isolation, cDNA was prepared for gene expression analysis using a RT2 Profiler PCR expression array. This custom designed qPCR expression array contained genes for opioid peptides and receptors, as well as genes involved in stress-responses and candidate genes involved in synaptic plasticity, including those upregulated following oxycodone self-administration in mice. Few sex differences are seen in hippocampal gene expression in control (unstressed) rats. In response to CIS, gene expression in the hippocampus was altered in males but not females. In males, opioid, stress, plasticity and kinase/signaling genes were all down-regulated following CIS, except for the gene that codes for corticotropin releasing hormone, which was upregulated. Changes in opioid gene expression following chronic stress were limited to the CA2 and CA3 regions (lateral sample). In conclusion, modest sex- and regional-differences are seen in expression of the opioid receptor genes, as well as genes involved in stress and plasticity responses in the hippocampus following CIS. Highlights • Unstressed female rats have less Arc expression in hippocampus than males. • Chronic immobilization stress (CIS) down-regulates opioid gene expression in males. • CIS up-regulates Crh but down-regulates other stress genes in male hippocampi. • CIS down-regulates Arc and other plasticity genes in male hippocampi. • CIS down-regulates select kinases and signaling molecules in male hippocampi. |
Databáze: | OpenAIRE |
Externí odkaz: |