Localization and distribution of nickel and other elements in in-vitro grown Alyssum corsicum exhibiting morphological changes in trichomes: initial insights into molecular mechanisms of nickel hyperaccumulation
| Autor: | Nezaket Adigüzel, Danika Leduc, Seniha Selcen Babaoğlu Aydaş, Leyla Açık, Şeküre Şebnem Ellialtioğlu, Yusuf Kağan Kadioğlu, Zekiye Suludere |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Key words: Alyssum corsicum
polarized energy dispersive X-ray fluorescence antioxidant enzymes differentially expressed genes fungi food and beverages Plant Science Biology Trichome Phytoremediation chemistry.chemical_compound Murashige and Skoog medium chemistry Guard cell Botany Shoot Hyperaccumulator Sodium dodecyl sulfate Polyacrylamide gel electrophoresis |
| Zdroj: | Volume: 37, Issue: 6 1115-1124 Turkish Journal of Botany |
| ISSN: | 1300-008X 1303-6106 |
| Popis: | Alyssum corsicum Duby is a candidate plant used for phytomining and phytoremediation studies with its high Ni-accumulating ability of the aboveground tissues. Metal localization and concentration by hyperaccumulator plants and the physiological basis of these phenomena have been of great interest in recent years. A. corsicum seeds were grown at different concentrations of Ni in Murashige and Skoog medium. Multielement concentrations of the plant roots and shoots were determined by polarized energy dispersive X-ray fluorescence. Ni, Ca, Mg, and Fe localization and concentration at the upper and lower leaf surfaces were determined by scanning electron microscopy coupled with the energy-dispersive X-ray analysis technique (SEM-EDX). Results showed that Ni is accumulated more by the shoots than the roots of the plant and that Ni is concentrated mainly in the trichome and the stoma guard cells of the leaves. Trichome density on the leaves was reduced, and some morphological changes of the trichome structure were also observed with increasing Ni levels. Analysis of antioxidant enzyme activities in roots and shoots was performed. The total leaf proteins of the plant were examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and differentially expressed genes were detected by specifically designed primers. |
| Databáze: | OpenAIRE |
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