Maternal environmental contribution to adult sensitivity and resistance to obesity in Long Evans rats

Autor: Aron Weller, Mariana Schroeder, Timothy H. Moran, Liat Shbiro
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Leptin
Male
Time Factors
lcsh:Medicine
Diabetes and Endocrinology/Obesity
0302 clinical medicine
Pregnancy
Nutrition/Obesity
Lactation
Diabetes and Endocrinology/Endocrinology
Insulin
lcsh:Science
2. Zero hunger
0303 health sciences
Neuroscience/Behavioral Neuroscience
Multidisciplinary
Alanine Transaminase
Neuroscience/Experimental Psychology
Animals
Suckling
Cholesterol
medicine.anatomical_structure
Adipose Tissue
Physiology/Integrative Physiology
Animal Nutritional Physiological Phenomena
Female
medicine.symptom
Research Article
medicine.medical_specialty
Offspring
Rats
Inbred OLETF
030209 endocrinology & metabolism
Weaning
Hyperphagia
Biology
03 medical and health sciences
Internal medicine
medicine
Animals
Rats
Long-Evans
Obesity
Triglycerides
030304 developmental biology
Estrous cycle
Body Weight
lcsh:R
Feeding Behavior
medicine.disease
Rats
Pregnancy Complications
Endocrinology
Nutrition/Eating Disorders
Animals
Newborn
lcsh:Q
Metabolic syndrome
Energy Intake
Weight gain
Zdroj: PLoS ONE, Vol 5, Iss 11, p e13825 (2010)
PLoS ONE
ISSN: 1932-6203
Popis: Background The OLETF rat is an animal model of early onset hyperphagia induced obesity, presenting multiple pre-obese characteristics during the suckling period. In the present study, we used a cross-fostering strategy to assess whether interactions with obese dams in the postnatal environment contributed to the development of obesity. Methodology On postnatal Day (PND)-1 OLETF and control LETO pups were cross-fostered to same or opposite strain dams. An independent ingestion test was performed on PND11 and a nursing test on PND18. Rats were sacrificed at weaning or on PND90, and plasma leptin, insulin, cholesterol, triglycerides and alanine aminotransferase (ALT) were assayed. Fat pads were collected and weighed and adipocyte size and number were estimated. Body weight and intake, as well as the estrous cycle of the female offspring were monitored. Principal Findings During the suckling period, the pups' phenotype was almost completely determined by the strain of the mother. However, pups independently ingested food according to their genotype, regardless of their actual phenotype. At adulthood, cross fostered males of both strains and LETO females were affected in regard of their adiposity levels in the direction of the foster dam. On the other hand, OLETF females showed almost no alterations in adiposity but were affected by the strain of the dams in parameters related to the metabolic syndrome. Thus, OLETF females showed reduced liver adiposity and circulating levels of ALT, while LETO females presented a disrupted estrous cycle and increased cholesterol and triglycerides in the long term. Conclusions The present study provides further support for the early postnatal environment playing a sex-divergent role in programming later life phenotype. In addition, it plays a more central role in determining the functioning of mechanisms involved in energy balance that may provide protection from or sensitivity to later life obesity and pathologies related to the metabolic syndrome.
Databáze: OpenAIRE
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