Effect of Collagen Peptide, Alone and in Combination with Calcium Citrate, on Bone Loss in Tail-Suspended Rats
| Autor: | Yanchuan Guo, Junli Liu, Jianing Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Gastric gavage
Tail medicine.medical_specialty Bovine collagen Ovariectomy Rat model Pharmaceutical Science chemistry.chemical_element 030209 endocrinology & metabolism Hindlimb Calcium tail-suspended rats Article Analytical Chemistry lcsh:QD241-441 Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine lcsh:Organic chemistry bone loss Oral administration Bone Density Internal medicine Drug Discovery medicine Animals Humans Femur Physical and Theoretical Chemistry 030304 developmental biology 0303 health sciences Collagen peptide Organic Chemistry X-Ray Microtomography Rats calcium citrate Bone Diseases Metabolic Endocrinology chemistry Hindlimb Suspension Chemistry (miscellaneous) Ovariectomized rat Molecular Medicine Cattle Collagen Peptides collagen peptide |
| Zdroj: | Molecules Volume 25 Issue 4 Molecules, Vol 25, Iss 4, p 782 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25040782 |
| Popis: | Oral administration of bovine collagen peptide (CP) combined with calcium citrate (CC) has been found to inhibit bone loss in ovariectomized rats. However, the protective effects of CP and CP&ndash CC against bone loss have not been investigated in a tail-suspension simulated microgravity (SMG) rat model. Adult Sprague-Dawley rats (n = 40) were randomly divided into five groups (n = 8): a control group with normal gravity, a SMG control group, and three SMG groups that underwent once-daily gastric gavage with CP (750 mg/kg body weight), CC (75 mg/kg body weight) or CP&ndash CC (750 and 75 mg/kg body weight, respectively) for 28 days. After sacrifice, the femurs were analyzed by dual-energy X-ray absorptiometry, three-point bending mechanical tests, microcomputed tomography, and serum bone metabolic markers. Neither CP nor CP&ndash CC treatment significantly inhibited bone loss in SMG rats, as assessed by dual-energy X-ray absorptiometry and three-point bending mechanical tests. However, both CP and CP&ndash CC treatment were associated with partial prevention of the hind limb unloading-induced deterioration of bone microarchitecture, as demonstrated by improvements in trabecular number and trabecular separation. CP&ndash CC treatment increased serum osteocalcin levels. Dietary supplementation with CP or CP&ndash CC may represent an adjunct strategy to reduce the risk of fracture in astronauts. |
| Databáze: | OpenAIRE |
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