Genetic evidence for the involvement of Dicer-like 2 and 4 as well as Argonaute 2 in the Nicotiana benthamiana response against Pelargonium line pattern virus
| Autor: | Miryam Pérez-Cañamás, Konstantina Katsarou, Carmen Hernández, Elizabeth Hevia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Ribonuclease III
AGO2 RNA virus Nicotiana benthamiana Virology Tombusviridae Tobacco Gene silencing RNA Viruses Antiviral RNA silencing Plant Diseases Plant Proteins RNA Double-Stranded DCL biology Pelargonium line pattern virus RNA Plant Argonaute biology.organism_classification Cell biology RNA silencing antiviral RNA silencing Argonaute Proteins biology.protein RNA Viral RNA Interference PLPV Dicer |
| Zdroj: | The Journal of General Virology Digital.CSIC. Repositorio Institucional del CSIC instname RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | [EN] In plants, RNA silencing functions as a potent antiviral mechanism. Virus-derived double-stranded RNAs (dsRNAs) trigger this mechanism, being cleaved by Dicer-like (DCL) enzymes into virus small RNAs (vsRNAs). These vsRNAs guide sequence-specific RNA degradation upon their incorporation into an RNA-induced silencing complex (RISC) that contains a slicer of the Argonaute (AGO) family. Host RNA dependent-RNA polymerases, particularly RDR6, strengthen antiviral silencing by generating more dsRNA templates from RISC-cleavage products that, in turn, are converted into secondary vsRNAs by DCLs. Previous work showed that Pelargonium line pattern virus (PLPV) is a very efficient inducer and target of RNA silencing as PLPV-infected Nicotiana benthamiana plants accumulate extraordinarily high amounts of vsRNAs that, strikingly, are independent of RDR6 activity. Several scenarios may explain these observations including a major contribution of dicing versus slicing for defence against PLPV, as the dicing step would not be affected by the RNA silencing suppressor encoded by the virus, a protein that acts via vsRNA sequestration. Taking advantage of the availability of lines of N. benthamiana with DCL or AGO2 functions impaired, here we have tried to get further insights into the components of the silencing machinery that are involved in anti-PLPV-silencing. Results have shown that DCL4 and, to lesser extent, DCL2 contribute to restrict viral infection. Interestingly, AGO2 apparently makes even a higher contribution in the defence against PLPV, extending the number of viruses that are affected by this particular slicer. The data support that both dicing and slicing activities participate in the host race against PLPV. This work was supported by grant BFU2015--70261 from the Ministerio de Economia y Competitividad (MINECO, Spain)--FEDER and PROMETEO/2019/012 from Generalitat Valenciana (GVA) (to C. H). M.P.-C. was the recipient of contracts from MINECO--FEDER and GVA, and E.H. was the recipient of a contract from MINECO--FEDER. K.K. was supported by the grant `Emblematic Action for Research in the Cretan Agrofood sector: Four Institutions, Four References' (AGRO4CRETE -2018S.01300000) held by the General Secretary for Research and Technology of Greece. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|