Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas
| Autor: | Antonino Carbone, Gianluca Gaidano, Kaarle Franssila, Manuela Bragardo, Vito L. Burgio, Roberto Cauda, Sara Bonissoni, Simona Zupo, Charles A. Janeway, Henrik Wolff, Donatella Buonfiglio, Valter Redoglia, Umberto Dianzani |
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| Rok vydání: | 1999 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Lymphoid Tissue T cell T-Lymphocytes Immunology CD1 Thymus Gland Biology Settore MED/17 - MALATTIE INFETTIVE Lymphocyte Activation Lymphoma T-Cell T cell lymphomas Mice T-Lymphocyte Subsets medicine Tumor Cells Cultured Immunology and Allergy Cytotoxic T cell Animals Humans IL-2 receptor Antigen-presenting cell activated T cells Sequence Homology Amino Acid ZAP70 Antibodies Monoclonal Cell Differentiation Natural killer T cell Molecular biology 3. Good health medicine.anatomical_structure Organ Specificity CD8 |
| Zdroj: | European journal of immunology. 29(9) |
| ISSN: | 0014-2980 |
| Popis: | We have previously characterized mouse H4 (mH4), a surface glycoprotein recognized by the C398.4A monoclonal antibody. We now show that C398.4A also binds its human putative homolog (hpH4). Both hpH4 and mH4 (1) are selectively expressed by activated T cells and mature thymocytes, (2) are disulfide-linked dimers of two chains (29/37 kDa in humans, 25/29 kDa in mice), whose N-deglycosylation produces a single band at 20 - 21 kDa, and (3) display a low association with CD4 and the TCR. The expression pattern of hpH4 and its biochemical features showed that it is different from other known activation molecules, and this was confirmed when analysis of the tryptic digest of the hpH4 29-kDa band by peptide mass searching using matrix-assisted laser desorption ionization mass spectrometry did not reveal any significant homology with other molecules. In normal lymphoid tissue, hpH4 is expressed by T cells located at the periphery of lymph node germinal centers and paracortical areas. In T cell neoplasia, expression of hpH4 clusters with a subset of peripheral T cell lymphomas with a large-cell component, and with cases of angioimmunoblastic T cell lymphomas. Overall, these data provide evidence for a novel T cell activation molecule that could help in the phenotypic categorization of T cell malignancies. |
| Databáze: | OpenAIRE |
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