Genetic interventions in mammalian cells; applications and uses in high-throughput screening and drug discovery
| Autor: | Anne I. Kinnaird, Sally L. Hampton |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Drug
Health Toxicology and Mutagenesis High-throughput screening media_common.quotation_subject Phenotypic screening Drug Evaluation Preclinical Gene Expression Computational biology Biology Toxicology Bioinformatics Cell Line Drug Discovery Animals Humans media_common Mammals Reverse pharmacology Genetic interventions Drug discovery Gene Transfer Techniques Cell Biology High-Throughput Screening Assays Gene Expression Regulation Genes Molecular targets Genetic Engineering Function (biology) |
| Zdroj: | Cell biology and toxicology. 26(1) |
| ISSN: | 1573-6822 |
| Popis: | Functional cellular assays are the bedrock of modern drug discovery. These utilise cellular systems that yield a measurable biochemical product or physiological response to drug stimulation. Often, these functional responses are studied by the introduction of the molecular target of choice into an inert cellular background to create a more discriminating system. There are as many techniques for delivery of the required target gene as there are techniques for studying their function. This article will consider the genetic modification of cell lines in vitro to develop cell-based assays for drug discovery and high-throughput screening. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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