Primary structure of solitary form of gonadotropin-releasing hormone (GnRH) in cichlid pituitary; three forms of GnRH in brain of cichlid and pumpkinseed fish
| Autor: | Richard C. Francis, Carol M. Warby, Minkyu Park, J.F.F. Powell, Russell D. Fernald, P. Licht, Nancy M. Sherwood, A. G. Craig, Jean Rivier, Stephanie A. White, Wolfgang H. Fischer |
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| Rok vydání: | 1995 |
| Předmět: |
endocrine system
medicine.medical_specialty Physiology Clinical Biochemistry Central nervous system Molecular Sequence Data Gonadotropin-releasing hormone Biology Biochemistry Pyroglutamate aminopeptidase Gonadotropin-Releasing Hormone Cellular and Molecular Neuroscience Endocrinology Cichlid Internal medicine medicine Animals Amino Acid Sequence Order perciformes Peptide sequence Chromatography High Pressure Liquid Protein primary structure Fishes biology.organism_classification medicine.anatomical_structure Pituitary Gland Fish hormones hormone substitutes and hormone antagonists |
| Zdroj: | Regulatory peptides. 57(1) |
| ISSN: | 0167-0115 |
| Popis: | GnRH is a decapeptide family with at least nine distinct structures. Vertebrates, except for most placental mammals, have more than one of these GnRH forms within the brain. We report chromatographical and immunological evidence that three forms of GnRH are in the brains of both cichlid (Haplochromis burtoni) and pumpkinseed (Lepomis gibbosus) fishes. We argue that the three forms correspond to those previously described as sea bream GnRH (sbGnRH), chicken GnRH-II and salmon GnRH. In contrast, only one GnRH form was present in the pituitary of the cichlid and is identified as sbGnRH by amino acid sequence. This is the first report in which the primary structure of GnRH is determined from pituitary tissue. The N-terminus was identified by monitoring the digestion of the peptide by pyroglutamate aminopeptidase with matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS). The amidation of the C-terminus was established using an esterification procedure for monitoring with MALDI-MS. This report supports the idea that three forms of GnRH within one species is widespread in the order Perciformes. The present study establishes sbGnRH as the third GnRH form in H. burtoni and predicts that sbGnRH is synthesized in preoptic neurons, then transported to the pituitary in the preoptic-hypophyseal axons for the release of one or both gonadotropins. |
| Databáze: | OpenAIRE |
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