Morphological, physiological and molecular biological characterisation of isolates from first cases of Acanthamoeba keratitis in Slovakia
| Autor: | Jozef Timko, Viera Nagyová, Arpád Nagy |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Slovakia Adolescent Genotype Sequence analysis Molecular Sequence Data Acanthamoeba Biology DNA Ribosomal Keratitis Microbiology Cornea RNA Ribosomal 18S medicine Animals Cluster Analysis Humans Ribosomal DNA Phylogeny Czech Republic General Veterinary Genes rRNA Sequence Analysis DNA General Medicine DNA Protozoan Middle Aged Ribosomal RNA medicine.disease biology.organism_classification Contact lens Infectious Diseases Acanthamoeba Keratitis Acanthamoeba keratitis Insect Science Female Parasitology RNA Protozoan |
| Zdroj: | Parasitology Research. 106:861-872 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-010-1731-3 |
| Popis: | Acanthamoeba keratitis (AK) is a relatively rare disease worldwide. Over the past 10 years, five cases of AK were reported in Slovakia. Four preserved Slovak strains and one strain from the Czech Republic isolated from corneal scrapes of patients with AK are characterised in this study. Genotype identification of isolates is based on sequences of the PCR amplimer GTSA.B1 amplified from 18S ribosomal DNA. A strain isolated from the first patient in 1999 was classified as a rare sequence type T15. This is just the second report in which genotype T15 has been associated with AK. The other three Slovak strains were identified as belonging to the most common genotype T4. The only strain originating from the Czech Republic was classified as sporadically appearing sequence type T3. All isolates were also studied for their temperature tolerance and growth characteristics. The cythopatic effect was tested in vitro on Vero cell cultures. |
| Databáze: | OpenAIRE |
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