Botulinum Toxin A Detrusor Injections Reduce Postsynaptic Muscular M2, M3, P2X2, and P2X3 Receptors in Children and Adolescents Who Have Neurogenic Detrusor Overactivity: A Single-blind Study
| Autor: | Gary W. Lawrence, Helmut H. Knispel, Jochen Neuhaus, Kurt Miller, Judith Priefert, Heinrich Schulte-Baukloh |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Adolescent Urology Down-Regulation Nerve fiber Injections Young Adult Postsynaptic potential Statistical significance Muscarinic acetylcholine receptor medicine Humans Single-Blind Method Prospective Studies Botulinum Toxins Type A Child Receptor Receptor Muscarinic M3 Receptor Muscarinic M2 Urinary bladder Dose-Response Relationship Drug Urinary Bladder Overactive business.industry Purinergic receptor Immunohistochemistry Urodynamics Treatment Outcome medicine.anatomical_structure Neuromuscular Agents Bladder augmentation Anesthesia Female business Receptors Purinergic P2X3 Follow-Up Studies Receptors Purinergic P2X2 |
| Zdroj: | Urology. 81:1052-1057 |
| ISSN: | 0090-4295 |
| DOI: | 10.1016/j.urology.2012.12.021 |
| Popis: | Objective To analyze the effect of OnabotulinumtoxinA detrusor injections on postsynaptic muscular receptors in children and adolescents with neurogenic detrusor overactivity. Materials and Methods A bladder augmentation became necessary in 10 children and adolescents (7 males, 3 females; median age, 12 years) who had neurogenic detrusor overactivity. Seven had previously received 1 to 8 (average 3.86) OnabotulinumtoxinA detrusor injections, but their detrusor pressure could not be maintained at tolerable levels because of low-compliance bladder. The last injection session had been completed an average of 3 months (range, 1.5-3.5 months) previously. Three patients had never received that therapy and were considered controls. On the bladder dome resections, a specific receptor analysis (muscarinic M2 and M3 and purinergic P2X1, P2X2, and P2X3) was performed with confocal immunofluorescence, and nerve fiber density was analyzed with light-microscopic 3,3′-diaminobenzidine–immunohistochemical staining. Results Receptor analysis showed a downregulation of all examined receptors after OnabotulinumtoxinA injections; the reductions in M2, M3, P2X2, and P2X3 receptors reached a significance level of P |
| Databáze: | OpenAIRE |
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