Store-Operated Calcium Entries Control Neural Stem Cell Self-Renewal in the Adult Brain Subventricular Zone

Autor: Valérie Coronas, Christophe Magaud, Florence Domenichini, Thomas Harnois, Elodie Terrié, Patricia Arnault, Bruno Constantin, Patrick Bois
Přispěvatelé: Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Nervous system
Stem cell-microenvironment interactions
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
Neurogenesis
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Subventricular zone
Stem cells
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Biology
03 medical and health sciences
Neural Stem Cells
Calcium flux
Neurosphere
medicine
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Animals
Calcium Signaling
Cell Self Renewal
Cell Proliferation
MESH: Adult Stem Cells
Animals
Cell Proliferation
Cell Self Renewal
Mice Inbred C57BL
Neural Stem Cells
Neurogenesis
Neurons
Brain
Calcium
Calcium Channels
Calcium Signaling
Neurons
Adult stem cells
Stem cell asymmetry
Brain
Cell Biology
Stem cell niche
Neural stem cell
3. Good health
Cell biology
Mice
Inbred C57BL
030104 developmental biology
Stem cell division
medicine.anatomical_structure
nervous system
Molecular Medicine
Self-renewal
Calcium
Calcium Channels
Stem cell
Neurogenesis/neural regeneration
Developmental Biology
Adult stem cell
Zdroj: STEM CELLS
STEM CELLS, AlphaMed Press, 2018, 36 (5), pp.761-774. ⟨10.1002/stem.2786⟩
ISSN: 1066-5099
DOI: 10.1002/stem.2786⟩
Popis: The subventricular zone (SVZ) is the major stem cell niche in the brain of adult mammals. Within this region, neural stem cells (NSC) proliferate, self-renew and give birth to neurons and glial cells. Previous studies underlined enrichment in calcium signaling-related transcripts in adult NSC. Because of their ability to mobilize sustained calcium influxes in response to a wide range of extracellular factors, store-operated channels (SOC) appear to be, among calcium channels, relevant candidates to induce calcium signaling in NSC whose cellular activities are continuously adapted to physiological signals from the microenvironment. By Reverse Transcription Polymerase Chain Reaction (RT-PCR), Western blotting and immunocytochemistry experiments, we demonstrate that SVZ cells express molecular actors known to build up SOC, namely transient receptor potential canonical 1 (TRPC1) and Orai1, as well as their activator stromal interaction molecule 1 (STIM1). Calcium imaging reveals that SVZ cells display store-operated calcium entries. Pharmacological blockade of SOC with SKF-96365 or YM-58483 (also called BTP2) decreases proliferation, impairs self-renewal by shifting the type of SVZ stem cell division from symmetric proliferative to asymmetric, thereby reducing the stem cell population. Brain section immunostainings show that TRPC1, Orai1, and STIM1 are expressed in vivo, in SOX2-positive SVZ NSC. Injection of SKF-96365 in brain lateral ventricle diminishes SVZ cell proliferation and reduces the ability of SVZ cells to form neurospheres in vitro. The present study combining in vitro and in vivo approaches uncovers a major role for SOC in the control of SVZ NSC population and opens new fields of investigation for stem cell biology in health and disease.
Databáze: OpenAIRE
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