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Kovaliov V. V. FEATURES OF ANTIOXIDANT-PROOXIDANT BALANCE OF THE MEDULLARY AREA UNDER THE INFLUENCE OF SKELETAL TRAUMA OF DIFFERENT SEVERITY COMPLICATED BY BLOOD LOSS. Актуальні проблеми транспортної медицини / Actual problems of transport medicine / 2018;4(54):126-134. ISSN 1818-9385 DOI http://dx.doi.org/10.5281/zenodo.2525700 http://aptm.org.ua FEATURES OF ANTIOXIDANT-PROOXIDANT BALANCE OF THE MEDULLARY AREA UNDER THE INFLUENCE OF SKELETAL TRAUMA OF DIFFERENT SEVERITY COMPLICATED BY BLOOD LOSS V. V. Kovaliov I.Horbachevsky Ternopil State Medical University Abstract Purpose: to find out the features of antioxidant-prooxidant balance of the medullary area under the influence of skeletal trauma of different severity, complicated by blood loss. Materials and Methods. Experiments were performed on 98 non-linear white male rats weighing 180-200 g. All animals were divided into 4 groups: control and three experimental groups. In the experimental group 1, a closed fracture of the thigh was modeled under thiopental-sodium anesthesia; in the group 2, blood loss of 20-22 % of total blood volume with the introduction of an autoblood into the abdominal cavity at a rate of 0.5 ml per 100 g of animal weight, was additionally precipitated; in the experimental group 3, in addition, the adjacent thigh was broken. The rats were withdrawn from the experiment in conditions of anxiety after 1, 3 and 7 days after the simulation of injuries by the method of total bloodletting from the heart. In the medullary area of the experimental animals, the content of TBC-active LPO products, the activity of catalase and the antioxidant-prooxidant index were calculated: catalase / TBC-active products of LPO. Results. Under conditions of an isolated skeletal trauma, the intensity of lipid peroxidation increases to the third day, and then goes down to the seventh day. The activity of catalase, which provides practically the same value of the antioxidant-prooxidant index in all terms of observation and indicates the adequacy of antioxidant secretion, varies accordingly. Additional blood loss is accompanied by a significant increase in the content of secondary lipoperoxidation products and a decrease in the activity of catalase from 1 to 7 days. The fracture of the adjacent thigh against the background of isolated skeletal trauma and blood loss worsens the course of traumatic illness, indicating that there is an increase in the disturbances of the studied parameters. However, their degree is lower than after modeling blood loss on the background of isolated skeletal trauma, indicating significant sensitivity of the medullary area to hypoxia and the leading role of blood loss in initiating lipid peroxidation processes and exhaustion of antioxidant defense in this area of a kidney. Conclusion. Simulation of severe skeletal trauma, complicated by blood loss, leads to significant accumulation in the medullary area the secondary lipid peroxidation products, depletion of the enzymatic level of antioxidant defense, antioxidant-prooxidant balance in relation to the predominance of prooxidant mechanisms that increase from the first to the seventh days of the post-traumatic period. Key words: kidney, medulla; skeletal trauma, blood loss, lipoperoxidation, antioxidant defense. |
