Effects of exogenous nitric oxide on growth, proline accumulation and antioxidant capacity in Cakile maritima seedlings subjected to water deficit stress
| Autor: | Kaouthar Saadallah, Marianne Bordenave, Chedly Abdelly, Arnould Savouré, Kilani Ben Rejeb, Asma Jday, Séverine Planchais, Inès Slama |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant biology medicine.medical_treatment Plant Science Malondialdehyde medicine.disease_cause 01 natural sciences Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Proline dehydrogenase Biochemistry chemistry Catalase biology.protein medicine Osmotic pressure Proline Food science Agronomy and Crop Science Oxidative stress 010606 plant biology & botany |
| Zdroj: | Functional plant biology : FPB. 43(10) |
| ISSN: | 1445-4416 |
| Popis: | Nitric oxide (NO) – an endogenous signalling molecule in plants and animals – mediates responses to biotic and abiotic stresses. In the present study, we examined the role of exogenous application of NO in mediating stress responses in Cakile maritima Scop. seedlings under water deficit stress using sodium nitroprusside (SNP) as NO donor and as a pre-treatment before the application of stress. Water deficit stress was applied by withholding water for 14 days. Growth, leaf water content (LWC), osmotic potential (ψs), chlorophyll, malondialdehyde (MDA), electrolyte leakage (EL), proline and Δ1-pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (ProDH) protein levels were determined. Enzyme activities involved in antioxidant activities (superoxide dismutase (SOD) and catalase (CAT)) were measured upon withholding water. The results showed that shoot biomass production was significantly decreased in plants subjected to water deficit stress alone. However, in water deficit stressed plants pre-treated with SNP, growth activity was improved and proline accumulation was significantly increased. Proline accumulation was concomitant with the stimulation of its biosynthesis as shown by the accumulation of P5CS proteins. Nevertheless, no significant change in ProDH protein levels was observed. Besides plants showed lower water deficit-induced lipid membrane degradation and oxidative stress after the pretreatment with 100 µM SNP. This behaviour was related to the increased activity of SOD and CAT. Thus, we concluded that NO increased C. maritima drought tolerance and mitigated damage associated with water deficit stress by the regulation of proline metabolism and the reduction of oxidative damage. |
| Databáze: | OpenAIRE |
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