BZU2/ZmMUTE controls symmetrical division of guard mother cell and specifies neighbor cell fate in maize

Autor: Chun Peng Song, Li Zuliang, Daojie Wang, Pengcheng Wang, David W. Galbraith, Shenglong Bai, Zhiyong Gao, Siyi Guo, Yusen Zhou, Jianfei Guo, Hongliang Wang, Qiao Xin
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Leaves
Cancer Research
Cell
Cell Communication
Plant Science
QH426-470
Plant Genetics
Database and Informatics Methods
0302 clinical medicine
Gene Expression Regulation
Plant
Guard cell
Arabidopsis
Basic Helix-Loop-Helix Transcription Factors
Plant Genomics
Asymmetric cell division
Cell Cycle and Cell Division
Promoter Regions
Genetic
Genetics (clinical)
Plant Proteins
0303 health sciences
biology
Stem Cells
Plant Anatomy
Cell Polarity
Eukaryota
Cell Differentiation
Genomics
Plants
Plants
Genetically Modified
Cell biology
medicine.anatomical_structure
Experimental Organism Systems
Cell Processes
Engineering and Technology
Sequence Analysis
Cell Division
Intracellular
Research Article
Biotechnology
Bioinformatics
Arabidopsis Thaliana
Bioengineering
Brassica
Cell fate determination
Research and Analysis Methods
Zea mays
03 medical and health sciences
Model Organisms
Plant and Algal Models
Sequence Motif Analysis
medicine
Genetics
Grasses
Molecular Biology
Transcription factor
Stomata
Ecology
Evolution
Behavior and Systematics
Actin
030304 developmental biology
Organisms
Biology and Life Sciences
Cell Biology
Stem Anatomy
biology.organism_classification
Maize
Seedlings
Mutation
Plant Stomata
Animal Studies
Plant Biotechnology
030217 neurology & neurosurgery
Zdroj: PLoS Genetics, Vol 15, Iss 8, p e1008377 (2019)
PLoS Genetics
ISSN: 1553-7404
1553-7390
Popis: Intercellular communication in adjacent cell layers determines cell fate and polarity, thus orchestrating tissue specification and differentiation. Here we use the maize stomatal apparatus as a model to investigate cell fate determination. Mutations in ZmBZU2 (bizui2, bzu2) confer a complete absence of subsidiary cells (SCs) and normal guard cells (GCs), leading to failure of formation of mature stomatal complexes. Nuclear polarization and actin accumulation at the interface between subsidiary mother cells (SMCs) and guard mother cells (GMCs), an essential pre-requisite for asymmetric cell division, did not occur in Zmbzu2 mutants. ZmBZU2 encodes a basic helix-loop-helix (bHLH) transcription factor, which is an ortholog of AtMUTE in Arabidopsis (BZU2/ZmMUTE). We found that a number of genes implicated in stomatal development are transcriptionally regulated by BZU2/ZmMUTE. In particular, BZU2/ZmMUTE directly binds to the promoters of PAN1 and PAN2, two early regulators of protodermal cell fate and SMC polarization, consistent with the low levels of transcription of these genes observed in bzu2-1 mutants. BZU2/ZmMUTE has the cell-to-cell mobility characteristic similar to that of BdMUTE in Brachypodium distachyon. Unexpectedly, BZU2/ZmMUTE is expressed in GMC from the asymmetric division stage to the GMC division stage, and especially in the SMC establishment stage. Taken together, these data imply that BZU2/ZmMUTE is required for early events in SMC polarization and differentiation as well as for the last symmetrical division of GMCs to produce the two GCs, and is a master determinant of the cell fate of its neighbors through cell-to-cell communication.
Author summary In the grasses, individual stomatal complexes comprise a pair of dumbbell-shaped guard cells associated with two subsidiary cells and the pore, which together play essential roles in the exchange of CO2 and O2, in xylem transport, and in transpiration. However, little is known about grass stomatal complex development. We have uncovered and characterized a key factor (BZU2/ZmMUTE) determining the formation of guard cells in Zea mays. Our data suggest that BZU2/ZmMUTE has a dual role, both as an important player in determining the formation of the guard mother cell, as well as being required for polarization and recruitment of the subsidiary mother cells.
Databáze: OpenAIRE
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