Efferocytosis capacities of blood monocyte‐derived macrophages in systemic sclerosis
| Autor: | Yu Augagneur, Laurent Vernhet, Valérie Lecureur, Patrick Jego, Claudie Morzadec, Alain Lescoat, Marie Lelong, Claire Cazalets, Olivier Fardel, Stéphane Jouneau, Alice Ballerie |
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| Přispěvatelé: | Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), CHU Pontchaillou [Rennes], This work was supported by the 'Groupe Francophone de la Recherche sur la Sclérodermie' (GFRS), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Integrin beta Chains [SDV]Life Sciences [q-bio] Immunology Inflammation medicine.disease_cause Jurkat cells Integrin β5 Monocytes Autoimmunity Apoptotic cell clearance 03 medical and health sciences Scavenger receptors 0302 clinical medicine Phagocytosis medicine Immunology and Allergy Humans Scavenger receptor Efferocytosis Receptor skin and connective tissue diseases Scleroderma Systemic Serine-Arginine Splicing Factors integumentary system business.industry Macrophages Cell Biology Scavenger Receptors Class B 030104 developmental biology Apoptosis Case-Control Studies Cancer research Systemic sclerosis Polarized Macrophage [SDV.IMM]Life Sciences [q-bio]/Immunology medicine.symptom business 030215 immunology |
| Zdroj: | Immunology and Cell Biology Immunology and Cell Biology, Nature Publishing Group, 2019, 97 (3), pp.340-347. ⟨10.1111/imcb.12217⟩ Immunology and Cell Biology, 2019, 97 (3), pp.340-347. ⟨10.1111/imcb.12217⟩ |
| ISSN: | 0818-9641 1440-1711 |
| DOI: | 10.1111/imcb.12217⟩ |
| Popis: | International audience; A defect in the apoptotic cell clearance (efferocytosis) by phagocytic cells may participate in autoimmunity and chronic inflammation. The mechanisms leading to the emergence of autoimmunity in systemic sclerosis (SSc) are still to be determined. In this study, the efferocytosis capacities of blood monocyte‐derived macrophages (MDM) from patients with SSc were evaluated. Blood monocytes obtained from patients with SSc and healthy donors (HD) were differentiated in vitro into macrophages. The capacities of MDM to engulf CFSE+ apoptotic Jurkat human T lymphocytes were compared between SSc MDM and HD using flow cytometry. The expression of classical engulfing receptors in SSc MDM and HD MDM was also evaluated and their involvement in the modulation of efferocytosis was confirmed using a siRNA approach. The mean phagocytic index (PI) reflecting efferocytosis capacities of SSc MDM (PI = 19.3 ± 3.0; n = 21) was significantly decreased in comparison with the PI of HD MDM (PI = 35.9 ± 3.0; n = 31; P < 0.001). In comparison with HD, SSc MDM exhibited a downregulated expression of scavenger receptor (SR)‐B1, SR‐A1 and integrin β5 (ITGβ5). In HD MDM, the extinction of these receptors was followed by a reduction of efferocytosis only for the repression of ITGβ5, suggesting a possible selective role of this integrin in the impaired efferocytosis observed in SSc. As efferocytosis may be at the crossroads of inflammation, autoimmunity and fibrosis, in showing impaired efferocytosis capacities of blood MDM in SSc, our study offers new pathogenesis considerations for the involvement of macrophages in the autoimmune processes driving this disorder. |
| Databáze: | OpenAIRE |
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