Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil
| Autor: | Severino Silvano dos Santos Higino, Sérgio Santos de Azevedo, Salomão Moreira de Figueiredo, Inácio José Clementino, Lara Borges Keid, Clebert José Alves, Silvio Arruda Vasconcellos, V.C.M. Rocha, C. S. A Batista |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Brucella ovis
Veterinary medicine Microbiological culture biology Short Communication Veterinary Microbiology lcsh:QR1-502 Brucella Ouchterlony double immunodiffusion biology.organism_classification Paraíba State Microbiology lcsh:Microbiology Serology law.invention ovine PCR OVINOCULTURA law Seroprevalence Ovis isolation Polymerase chain reaction |
| Zdroj: | Brazilian Journal of Microbiology, Volume: 41, Issue: 2, Pages: 365-367, Published: JUN 2010 Brazilian Journal of Microbiology, Vol 41, Iss 2, Pp 365-367 (2010) Brazilian Journal of Microbiology v.41 n.2 2010 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil. |
| Databáze: | OpenAIRE |
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