Hydrogen peroxide–induced oxidative stress impairs redox status and damages aerobic metabolism of breast muscle in broilers
| Autor: | Jiaolong Li, Zuodong Chen, Tong Xing, Yun Jiang, Feng Gao, Lin Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nrf2 signaling pathway
medicine.medical_specialty medicine.medical_treatment Intraperitoneal injection hydrogen peroxide antioxidant capacity medicine.disease_cause broiler Antioxidants Pectoralis Muscles Metabolism and Nutrition Random Allocation 03 medical and health sciences chemistry.chemical_compound Internal medicine Lactate dehydrogenase medicine Animals 030304 developmental biology lcsh:SF1-1100 chemistry.chemical_classification reactive oxygen species 0303 health sciences Chemistry Glutathione peroxidase 0402 animal and dairy science Broiler 04 agricultural and veterinary sciences General Medicine Metabolism Oxidants Malondialdehyde 040201 dairy & animal science Oxidative Stress Endocrinology Dietary Supplements glycolysis metabolism Animal Science and Zoology lcsh:Animal culture Chickens Oxidation-Reduction Anaerobic exercise Oxidative stress |
| Zdroj: | Poultry Science, Vol 100, Iss 2, Pp 918-925 (2021) Poultry Science |
| ISSN: | 0032-5791 |
| Popis: | Oxidative stress has always been a hot topic in poultry science. However, studies concerning the effects of redox status and glucose metabolism induced by hydrogen peroxide (H2O2) in the breast muscle of broilers have been rarely reported. This study was aimed to evaluate the impact of intraperitoneal injection of H2O2 on oxidative damage and glycolysis metabolism of breast muscle in broilers. We also explored the activation of the nuclear factor erythroid 2–related factor 2 (Nrf2) signaling pathway to provide possible mechanism of the redox imbalance. Briefly, a total of 320 one-day-old Arbor Acres chicks were randomly divided into 5 treatments with 8 replicates of 8 birds each (noninjected control, 0.75% saline-injected, 2.5, 5.0, and 10.0% H2O2-injected treatments). Saline group was intraperitoneally injected with physiological saline (0.75%) and H2O2 groups received an intraperitoneal injection of H2O2. The dosage of the injection was 1.0 mL/kg BW. All birds in the saline and H2O2 groups were injected on days 16 and 37 of the experimental period. At 42 d of age, 40 birds (8 cages per group and one chicken per cage) were selected to be stunned electrically (50 V, alternating current, 400 Hz for 5 s each one), and then immediately slaughtered via exsanguination. The results showed that broilers in the H2O2 injection group linearly exhibited higher contents of reactive oxygen species, carbonyl and malondialdehyde, and lower total antioxidant capacity and glutathione peroxidase activities. With the content of H2O2 increased, the H2O2 groups linearly downregulated the mRNA expressions of GPX, CAT, HMOX1, NQO1, and Nrf2 and its downstream target genes. In addition, H2O2 increased serum activities of creatine kinase and lactate dehydrogenase. Meanwhile, in the pectoral muscle, the glycogen content was linearly decreased, and the lactate content was linearly increased in muscle of broilers injected with H2O2. In addition, the activities of glycolytic enzymes including pyruvate kinase, hexokinase, and lactate dehydrogenase were linearly increased after exposure to H2O2. In conclusion, H2O2 injection could impair antioxidant status and enhance anaerobic metabolism of breast muscle in broilers. |
| Databáze: | OpenAIRE |
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