Cas12a-Activated Universal Field-Deployable Detectors for Bacterial Diagnostics

Autor:	Yue Li, Kaixiang Zhang, Xiaolin Ge, Hong Liu, Yi Wan, Yun Sun, Fengge Song, Yuanyuan Shen, Chaoyang Li, Jinghong Li, Aimin Wang, Xingmei Huang
Rok vydání:	2020
Předmět:	Pathogen detection Shigella dysenteriae Fluorophore biology Chemistry General Chemical Engineering Detector General Chemistry Computational biology biology.organism_classification Article Highly sensitive chemistry.chemical_compound Disease biomarker QD1-999 DNA
Zdroj:	ACS Omega, Vol 5, Iss 24, Pp 14814-14821 (2020) ACS Omega
ISSN:	2470-1343
DOI:	10.1021/acsomega.0c01911
Popis:	Field-deployable detectors of disease biomarkers provide a simple and fast analysis of clinical specimens. However, most of the existing field-deployable diagnostics have poor sensitivity and are not suitable for the detection of biomarkers with low abundance. Herein, we report a highly sensitive and rapid colorimetric readout paper-based assay for pathogen detection by integrating the unique collateral activity of a Cas12a-activated universal field-deployable detector (CUFD). The collateral effect of Cas12a results in a nonspecific destruction of a fluorophore biotin-labeled ssDNA reporter for the CUFD. This technique can quantify seven different kinds of pathogens in blood samples without any purification procedure, with sensitivity as low as 10 aM for the Shigella dysenteriae DNA. This CUFD technique has significant potential for the detection of pathogenic DNA as well as other types of DNA or RNA targets at the point-of-care application.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16fdc1aa29bf7fe58edd23ba7e8bce35 https://doi.org/10.1021/acsomega.0c01911 Zobrazit plný text záznamu