Dynamics of DOT1L localization and H3K79 methylation during meiotic prophase I in mouse spermatocytes
| Autor: | Liisa Kauppi, David Ontoso, Scott Keeney, Pedro A. San-Segundo |
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| Přispěvatelé: | Consejo Superior de Investigaciones Científicas (España), EMBO, National Institutes of Health (US), Ministerio de Economía y Competitividad (España), Junta de Castilla y León, Fundación Ramón Areces |
| Předmět: |
Male
Time Factors Heterochromatin H3K79 Biology Methylation Models Biological Article Histones Meiotic Prophase I Mice Meiosis Spermatocytes Genetics Homologous chromosome Animals Humans Spermatogenesis Genetics (clinical) Cell Nucleus Sex Chromosomes Lysine DOT1L Histone-Lysine N-Methyltransferase Methyltransferases Chromatin Mice Inbred C57BL Synaptonemal complex Protein Transport Histone Mutation biology.protein Histone modification |
| Zdroj: | University of Helsinki Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2012-3574 |
| Popis: | PMCID: PMC3969405 During meiotic prophase I, interactions between maternal and paternal chromosomes, under checkpoint surveillance, establish connections between homologs that promote their accurate distribution to meiotic progeny. In human, faulty meiosis causes aneuploidy resulting in miscarriages and genetic diseases. Meiotic processes occur in the context of chromatin; therefore, histone post-translational modifications are expected to play important roles. Here, we report the cytological distribution of the evolutionarily conserved DOT1L methyltransferase and the different H3K79 methylation states resulting from its activity (mono-, di- and tri-methylation; H3K79me1, me2 and me3, respectively) during meiotic prophase I in mouse spermatocytes. In the wild type, whereas low amounts of H3K79me1 are rather uniformly present throughout prophase I, levels of DOT1L, H3K79me2 and H3K79me3 exhibit a notable increase from pachynema onwards, but with differential subnuclear distribution patterns. The heterochromatic centromeric regions and the sex body are enriched for H3K79me3. In contrast, H3K79me2 is present all over the chromatin, but is largely excluded from the sex body despite the accumulation of DOT1L. In meiosis-defective mouse mutants, the increase of DOT1L and H3K79me is blocked at the same stage where meiosis is arrested. H3K79me patterns, combined with the cytological analysis of the H3.3, γH2AX, macroH2A and H2A.Z histone variants, are consistent with a differential role for these epigenetic marks in male mouse meiotic prophase I. We propose that H3K79me2 is related to transcriptional reactivation on autosomes during pachynema, whereas H3K79me3 may contribute to the maintenance of repressive chromatin at centromeric regions and the sex body. © 2013 Springer-Verlag. DO was supported by a predoctoral fellowship (JAE-Predoc) from the CSIC (Spain) and an EMBO short-term fellowship. Research was supported by NIH grant R01 GM105421 (to Maria Jasin and SK) and grants from MINECO (BFU2012-35748), Junta de Castilla y León (CSI025A11-2), and Fundación Ramón Areces to PSS. |
| Databáze: | OpenAIRE |
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