Quantification of Substitution of Gelatin Methacryloyl: Best Practice and Current Pitfalls
| Autor: | Alexander Southan, Christiane Claaßen, Günter E. M. Tovar, Marc H. Claaßen, Kirsten Borchers, Vincent Truffault, Lisa Sewald |
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| Rok vydání: | 2017 |
| Předmět: |
1h nmr spectroscopy
food.ingredient Polymers and Plastics Proton Magnetic Resonance Spectroscopy Biocompatible Materials Bioengineering 02 engineering and technology 010402 general chemistry Methacrylate 01 natural sciences Gelatin Biomaterials chemistry.chemical_compound food Materials Chemistry Methacrylamide Carbon-13 Magnetic Resonance Spectroscopy Reference standards Chromatography Tissue Engineering Tissue Scaffolds Acetylation Reference Standards 021001 nanoscience & nanotechnology 0104 chemical sciences NMR spectra database Cross-Linking Reagents chemistry Proton NMR Methacrylates 0210 nano-technology Two-dimensional nuclear magnetic resonance spectroscopy |
| Zdroj: | Biomacromolecules. 19:42-52 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.7b01221 |
| Popis: | Cross-linkable gelatin methacryloyl (GM) is widely used for the generation of artificial extracellular matrix (ECM) in tissue engineering. However, the quantification of modified groups in GM is still an unsolved issue, although this is the key factor for tailoring the physicochemical material properties. In this contribution, 1H–13C-HSQC NMR spectra are used to gain detailed structural information on GMs and of 2-fold modified gelatin containing methacryloyl and acetyl groups (GMAs). Distinctive identification of methacrylate, methacrylamide, and acetyl groups present in GMs and GMAs revealed an overlap of methacrylamide and modified hydroxyproline signals in the 1H NMR spectrum. Considering this, we suggest a method to quantify methacrylate and methacrylamide groups in GMs precisely based on simple 1H NMR spectroscopy with an internal standard. Quantification of acetylation in GMAs is also possible, yet, 2D NMR spectra are necessary. The described methods allow direct quantification of modified groups i... |
| Databáze: | OpenAIRE |
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