Improving the organic solvent resistance of lipase a from Bacillus subtilis in water-ethanol solvent through rational surface engineering
| Autor: | Hee Taek Kim, Jin Suk Lee, Ye Jean Jung, Kyoungseon Min, Siseon Lee, Young Je Yoo, Si Jae Park, Jeong Chan Joo |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Environmental Engineering Mutagenesis (molecular biology technique) Bioengineering Bacillus subtilis 010501 environmental sciences 01 natural sciences chemistry.chemical_compound 010608 biotechnology Enzyme Stability Organic chemistry Lipase Waste Management and Disposal 0105 earth and related environmental sciences chemistry.chemical_classification Ethanol biology Renewable Energy Sustainability and the Environment Water General Medicine Protein engineering biology.organism_classification Solvent Enzyme chemistry Biocatalysis biology.protein Solvents |
| Zdroj: | Bioresource technology. 337 |
| ISSN: | 1873-2976 |
| Popis: | Given that lipase is an enzyme applicable in various industrial fields and water-miscible organic solvents are important reaction media for developing industrial-scale biocatalysis, a structure-based strategy was explored to stabilize lipase A from Bacillus subtilis in a water–ethanol cosolvent. Site-directed mutagenesis of ethanol-interacting sites resulted in 4 mutants, i.e., Ser16Gly, Ala38Gly, Ala38Thr, and Leu108Asn, which were stable in 50% ethanol and had up to 1.8-fold higher stability than the wild-type. In addition, Leu108Asn was more thermostable at 45 °C than the wild type. The results discussed in this study not only provide insights into strategies for enzyme engineering to improve organic solvent resistance but also suggest perspectives on pioneering routes for constructing enzyme-based biorefineries to produce value-added fuels and chemicals. |
| Databáze: | OpenAIRE |
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