Elf5 and Ets2 maintain the mouse extraembryonic ectoderm in a dosage dependent synergistic manner
| Autor: | Peter L. Pfeffer, Ric Broadhurst, Martyn Donnison |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Heterozygote
Plac1 Time Factors Ets2 Mutant Ectoderm Biology Proto-Oncogene Protein c-ets-2 Animals Genetically Modified Mice Gene expression medicine Animals RNA Small Interfering Molecular Biology Gene Transcription factor Alleles Oligonucleotide Array Sequence Analysis Genetics Ectoplacental cone Gene Expression Profiling Trophoblast Gene Expression Regulation Developmental Cell Differentiation Heterozygote advantage Extraembryonic ectoderm Cell Biology Fibroblasts Elf5 Phenotype Cell biology DNA-Binding Proteins medicine.anatomical_structure Spry4 Mutation Cattle Transcription Factors Developmental Biology |
| Zdroj: | Developmental Biology. 397:77-88 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2014.10.011 |
| Popis: | The ETS superfamily transcription factors Elf5 and Ets2 have both been implicated in the maintenance of the extraembryonic ectoderm (ExE) of the mouse embryo. While homozygous mutants of either gene result in various degrees of ExE tissue loss, heterozygotes are without phenotype. We show here that compound heterozygous mutants exhibit a phenotype intermediate to that of the more severe Elf5−/− and the milder Ets2−/− mutants. Functional redundancy is shown via commonalities in expression patterns, in target gene expression, and by partial rescue of Elf5−/− mutants through overexpressing Ets2 in an Elf5-like fashion. A model is presented suggesting the functional division of the ExE region into a proximal and distal domain based on gene expression patterns and the proximal to distal increasing sensitivity to threshold levels of combined Elf5 and Ets2 activity. |
| Databáze: | OpenAIRE |
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