An alternative transcript of the FOG-2 gene encodes a FOG-2 isoform lacking the FOG repression motif
| Autor: | Eric C. Svensson, Rodney M. Dale, Benjamin F. Remo |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Transcriptional Activation Gene isoform genetic structures Blotting Western Molecular Sequence Data Biophysics Repressor Biology Transfection Biochemistry Article Mice Transcription (biology) Animals Protein Isoforms RNA Messenger Molecular Biology Transcription factor Psychological repression Genetics Binding Sites Reverse Transcriptase Polymerase Chain Reaction GATA4 Gene Expression Profiling Alternative splicing Promoter Cell Biology GATA4 Transcription Factor DNA-Binding Proteins Repressor Proteins Alternative Splicing NIH 3T3 Cells Protein Binding Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 357:683-687 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.04.008 |
| Popis: | The FOG family of transcriptional co-factors is composed of two members in mammals: FOG-1 and FOG-2. Both have been shown to bind to GATA factors and function as transcriptional co-repressors in specific cell and promoter contexts. We have previously defined a novel repression domain localized to the N-terminus of each FOG family member, the FOG Repression Motif, which is necessary for FOG-mediated transcriptional repression. In this report, we describe the identification and characterization of a novel isoform of FOG-2 lacking the FOG Repression Motif. In contrast to full-length FOG-2, this isoform is expressed predominately in the embryonic and adult heart. It can bind GATA4 avidly, but is unable to repress GATA4-mediated activation of cardiac-restricted gene promoters. Together, these results suggest that FOG-2 repressive activity may be modulated by the generation of isoforms of FOG-2 lacking the FOG repression motif. |
| Databáze: | OpenAIRE |
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