AIDS and non-AIDS diffuse large B-cell lymphomas express different antigen profiles
| Autor: | K. H. Ramesh, Dongsheng Xu, Linda Cannizaro, P. Ajit Kumar, Robert M. Gormley, Rashna Madan, Alina Dulau, Ecaterina F. Tamas, Joseph A. Sparano, Pritish K. Bhattacharyya, Daniel Walsh, Harry L. Ioachim, Aaron J. LeValley, Howard Ratech, Xiaonan Xue |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Epstein-Barr Virus Infections Herpesvirus 4 Human Pathology medicine.medical_specialty Lymphoma B-Cell Translocation Genetic Pathology and Forensic Medicine Proto-Oncogene Proteins c-myc Immunophenotyping medicine Humans In Situ Hybridization In Situ Hybridization Fluorescence B cell Aged Lymphoma AIDS-Related Aged 80 and over Tissue microarray biology CD44 Germinal center Middle Aged Prognosis medicine.disease BCL6 Immunohistochemistry Survival Analysis Phenotype DNA-Binding Proteins Ki-67 Antigen medicine.anatomical_structure Interferon Regulatory Factors Proto-Oncogene Proteins c-bcl-6 biology.protein Female Neprilysin Lymphoma Large B-Cell Diffuse Diffuse large B-cell lymphoma |
| Zdroj: | Modern Pathology. 19:438-446 |
| ISSN: | 0893-3952 |
| DOI: | 10.1038/modpathol.3800493 |
| Popis: | Based on gene expression profiling, diffuse large B-cell lymphomas arising in immunocompetent patients can be divided into germinal center and activated B-cell types. Since little is known about acquired immunodeficiency syndrome associated diffuse large B-cell lymphomas, we tested whether the protein expression of germinal center and activated B-cell markers differed between acquired immunodeficiency syndrome (AIDS) vs non-AIDS diffuse large B-cell lymphomas. We immunohistochemically stained tissue microarrays of 39 de novo diffuse large B-cell lymphomas: 12 AIDS associated and 27 non-AIDS, with germinal center (BCL6, CD10, CyclinH) and activated B-cell markers (MUM1, CD138, PAK1, CD44, BCL2). We scored each case for percent positive cells (0–19% ¼ 0; 20–49% ¼ 1; 50–100% ¼ 2). The activated B-cell and germinal center summation scores of each case were used as (x, y) coordinate data points to construct two-dimensional contour-frequency plots. The contour plot of non-AIDS diffuse large B-cell lymphomas showed two distinct clusters: a cluster with a high germinal center phenotype (cluster 1) and a cluster with a high activated B-cell phenotype (cluster 3). In contrast, the AIDS-related diffuse large B-cell lymphomas formed a single aggregate (cluster 2) (P ¼ 0.02, Fisher exact test). When the contour plots of the AIDS-related and the non-AIDS cases were superimposed, cluster 2 of the AIDS cases expressed an intermediate germinal center/activated B-cell phenotype compared to clusters 1 and 3 of the non-AIDS diffuse large B-cell lymphomas. Our results confirm that non-AIDS diffuse large B-cell lymphomas segregate into two groups with either germinal center or activated B-cell phenotype. We report the new finding that the AIDS status of the patient predicts the immunophenotype of the diffuse large B-cell lymphomas. Modern Pathology (2006) 19, 438–446. doi:10.1038/modpathol.3800493; published online 27 January 2006 |
| Databáze: | OpenAIRE |
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