Traditional Horticulture Practices Increase the Production of Selected Withanolides in Withania Somnifera (L.) Dunal—A RP-UFLC Analysis

Autor:	Gireesh M. Ankad, Sandeep R. Pai, Jagadishchandra Hiremath, Harsha V. Hegde
Rok vydání:	2020
Předmět:	India Withania Withania somnifera Plant Roots 01 natural sciences Plant life Analytical Chemistry chemistry.chemical_compound Limit of Detection Statistical analysis Withanolides Chromatography High Pressure Liquid Chromatography Reverse-Phase Farmyard manure biology Plant Extracts 010405 organic chemistry Chemistry 010401 analytical chemistry Reproducibility of Results Agriculture General Medicine biology.organism_classification 0104 chemical sciences Inorganic fertilizer Plant Leaves Horticulture Withanolide Withaferin A Linear Models
Zdroj:	Journal of Chromatographic Science. 58:899-906
ISSN:	1945-239X 0021-9665
DOI:	10.1093/chromsci/bmaa057
Popis:	The study evaluates the effect of two traditional horticulture treatments mentioned in Vrikshayurveda, a text from ancient India on the science of plant life, namely Kunapa jala (KJ) and Pancha gavya (PG) on the production of Withaferin A (WFA), withanolide A (WIA) and Withanolide B (WIB) in Withania somnifera (L) Dunal. Leaves and roots of W. somnifera were collected from different treated groups viz. control, KJ, PG, farmyard manure (FYM) and inorganic fertilizer (NPK). Reverse phase ultra-flow liquid chromatography (RP-UFLC) method was developed, validated for simultaneous detection of WFA, WIA and WIB. Statistical analysis of data was performed by ANOVA and tested for significance by the Dunnett multiple comparison test and data were expressed as mean ± standard deviation (SD). Results revealed, leaves possessed highest WFA content and roots possessed highest content of WIA and WIB. PG treated leaves were observed highest WFA (18.29 mg/g) and roots were observed highest WIA (19.63 mg/g) and WIB (1.36 mg/g). Conclusively, RP-UFLC method for simultaneous detection of withanolides has been developed and validated to evaluate the effect of traditional horticulture treatments. It is concluded that the enhanced production of withanolides can be achieved by the application of PG when compared to NPK application.
Databáze:	OpenAIRE
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