Characterization of the capsular polysaccharide of Burkholderia (Pseudomonas) pseudomallei 304b
| Autor: | Hussein Masoud, May Ho, Tineke Schollaardt, Malcolm B. Perry |
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| Rok vydání: | 1997 |
| Předmět: |
Models
Molecular Bacterial capsule Burkholderia pseudomallei Magnetic Resonance Spectroscopy Melioidosis Molecular Sequence Data Methylation Microbiology Gas Chromatography-Mass Spectrometry Serology Antigen medicine Humans Molecular Biology Bacterial Capsules Antigens Bacterial biology Pseudomonas medicine.disease biology.organism_classification Antibodies Bacterial Virology Burkholderia Carbohydrate Sequence biology.protein Antibody Research Article |
| Zdroj: | Journal of Bacteriology. 179:5663-5669 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.179.18.5663-5669.1997 |
| Popis: | Burkholderia (Pseudomonas) pseudomallei is the causative agent of melioidosis, a bacterial infection of considerable morbidity in areas of endemicity of Southeast Asia and northern Australia. Clinical isolates of B. pseudomallei have been demonstrated to produce a lipopolysaccharide (LPS) containing two separate and chemically distinct antigenic O polysaccharides against which infected patients produced antibodies. A putative capsular polysaccharide (CPS) has also been reported and is thought to be antigenically conserved based on results of serological studies with clinical B. pseudomallei isolates. In the present study, the CPS isolated from B. pseudomallei 304b from northeastern Thailand was found to have an [alpha]D of +99 degrees (water), was composed of D-galactose (D-Gal), 3-deoxy-D-manno-2-octulosonic acid (KDO), and O-acetyl 3:1:1), and was a linear unbranched polymer of repeating tetrasaccharide units having the following structure: -3)-2-O-Ac-beta-D-Galp-(1-4)-alpha-D-Galp-(1-3)-beta-D -Galp-(1-5)-beta-D-KDOp-(2-. Sera from 13 of 15 patients with different clinical manifestations of melioidosis but not normal controls recognize the CPS, which suggests that it is immunogenic and raises the possibility that it may have a role as a vaccine candidate and/or diagnostic agent. |
| Databáze: | OpenAIRE |
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