Distinct roles of HF-1b/Sp4 in ventricular and neural crest cells lineages affect cardiac conduction system development
| Autor: | Jonathan T. Lu, Yusu Gu, Masahiko Hoshijima, Tara R St Amand, John J. Ross, Pilar Ruiz-Lozano, Monica Zamora, Janelle Stricker, Jonathan A. Epstein, Kenneth R. Chien |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Cell type Heart Ventricles Cell Sp4 Transcription Factor Heart failure Biology Tropomyosin receptor kinase C Connexins 03 medical and health sciences Mice Cardiac development 0302 clinical medicine Heart Conduction System Internal medicine medicine Myocyte Animals Cell Lineage Myocytes Cardiac Receptor trkC Molecular Biology Gene knockout 030304 developmental biology Mice Knockout 0303 health sciences Neural crest Cell Biology Cell biology Endocrinology medicine.anatomical_structure Phenotype Neural Crest Conduction system biology.protein Atrioventricular Node Electrical conduction system of the heart 030217 neurology & neurosurgery Neurotrophin Developmental Biology |
| Zdroj: | Developmental biology. 291(2) |
| ISSN: | 0012-1606 |
| Popis: | The heterogeneous cell types of the cardiac conduction system are responsible for coordinating and maintaining rhythmic contractions of the heart. While it has been shown that the cells of the conduction system are derived from myocytes, additional cell types, including neural crest cells, may play a role in the development and maturation of these specialized cell lineages. Previous work has shown that the expression of the hf-1b gene is required for specification of the cardiac conduction system. Using Cre-Lox technology, we conditionally mutated the hf-1b gene in the ventricular and the neural crest cell lineages. Cx40 immunohistochemistry on HF-1b tissue-restricted knockouts revealed a requirement for HF-1b in the cardiomyogenic lineage. Electrophysiological studies identified a second requirement for HF-1b in the neural crest-derived cells. Absence of HF-1b in the neural crest led to atrial and atrioventricular dysfunction resulting from deficiencies in the neurotrophin receptor trkC. Therefore, in this study, we document that a single transcription factor, HF-1b, acts through two separate cell types to direct distinct functions of the cardiac conduction system. |
| Databáze: | OpenAIRE |
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