Acetone-Butanol-Ethanol (ABE) production in fermentation of enzymatically hydrolyzed cassava flour by Clostridium beijerinckii BA101 and solvent separation
| Autor: | Leonardo Lépiz-Aguilar, María Laura Arias, Giselle Lutz, Carlos E. Rodríguez-Rodríguez |
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| Rok vydání: | 2013 |
| Předmět: |
Acidogenesis
Manihot Butanols Flour Chemical Fractionation Applied Microbiology and Biotechnology Acetone chemistry.chemical_compound Azeotropic distillation Bioreactor Food science Clostridium beijerinckii Downstream processing biology Ethanol Butanol General Medicine biology.organism_classification chemistry Biochemistry Fermentation Solvents Biotechnology |
| Zdroj: | Journal of microbiology and biotechnology. 23(8) |
| ISSN: | 1738-8872 |
| Popis: | Cassava constitutes an abundant substrate in tropical regions. The production of butanol in ABE fermentation by Clostridium beijerinckii BA101 using cassava flour (CF) was scaled-up to bioreactor level (5 L). Optimized fermentation conditions were applied; that is, 40oC, 60 g/l CF, and enzymatic pretreatment of the substrate. The batch fermentation profile presented an acidogenic phase for the first 24 h and a solventogenic phase afterwards. An average of 37.01 g/l ABE was produced after 83 h, with a productivity of 0.446 g/l/h. Butanol production was 25.71 g/l with a productivity of 0.310 g/l/h, high or similar to analogous batch processes described for other substrates. Solvent separation by different combinations of fractioned and azeotropic distillation and liquid-liquid separation were assessed to evaluate energetic and economic costs in downstream processing. Results suggest that the use of cassava as a substrate in ABE fermentation could be a cost-effective way of producing butanol in tropical regions. |
| Databáze: | OpenAIRE |
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