Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling
| Autor: | Konstantin V. Trusov, Andrei V. Chernyshev, Anastasiya I. Konokhova, Darya Y. Orlova, Stanislav Kozubek, Eva Bártová, Irina V. Khalo, Valeri P. Maltsev, Maxim A. Yurkin |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Statistics and Probability Kinetics Apoptosis Time-Lapse Imaging General Biochemistry Genetics and Molecular Biology law.invention Nuclear morphology 03 medical and health sciences Imaging Three-Dimensional Prophase Confocal microscopy law DNA Packaging Humans Cell Nucleus Microscopy Confocal General Immunology and Microbiology Kinetic model Chemistry Applied Mathematics Hep G2 Cells General Medicine Models Theoretical Chromatin 030104 developmental biology Modeling and Simulation Hepg2 cells Organelle Size Biophysics sense organs Single-Cell Analysis General Agricultural and Biological Sciences |
| Zdroj: | Journal of Theoretical Biology. 454:60-69 |
| ISSN: | 0022-5193 |
| DOI: | 10.1016/j.jtbi.2018.05.034 |
| Popis: | The dynamics of nuclear morphology changes during apoptosis remains poorly investigated and understood. Using 3D time-lapse confocal microscopy we performed a study of early-stage apoptotic nuclear morphological changes induced by etoposide in single living HepG2 cells. These observations provide a definitive evidence that nuclear apoptotic volume decrease (AVD) is occurring simultaneously with peripheral chromatin condensation (so called "apoptotic ring"). In order to describe quantitatively the dynamics of nuclear morphological changes in the early stage of apoptosis we suggest a general molecular kinetic model, which fits well the obtained experimental data in our study. Results of this work may clarify molecular mechanisms of nuclear morphology changes during apoptosis. |
| Databáze: | OpenAIRE |
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