Novel polymer blends for the preparation of membranes for biohybrid liver systems
| Autor: | Barbara Seifert, Guenter Malsch, Wolfgang Albrecht, George Altankov, Andreas Lendlein, Thomas Groth, Aneliya Kostadinova |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Biocompatibility Membrane permeability Cell Survival Polymers Surface Properties Biomedical Engineering Biophysics Synthetic membrane Acrylic Resins Bioengineering Biocompatible Materials 7-Alkoxycoumarin O-Dealkylase Microscopy Atomic Force law.invention Cell Line Biomaterials chemistry.chemical_compound law Polymer chemistry Materials Testing Cell Adhesion Humans ddc:610 Cell Proliferation Bioartificial liver device Membranes Artificial Liver Artificial Fibronectins Membrane chemistry Chemical engineering Permeability (electromagnetism) Hepatocytes Polymer blend Acrylonitrile Sulfonic Acids |
| Zdroj: | Kostadinova, A.; Seifert, B.; Albrecht, W.; Malsch, G.; Groth, T.; Lendlein, A.; Altankov, G.: Novel Polymer Blends for the Preparation of Membranes for Biohybrid Liver Systems . In: Journal of Biomaterials Science-Polymer Edition. Vol. 20 (2009) 5-6, 821-839. (DOI: 10.1163/156856209X427005) |
| ISSN: | 0920-5063 |
| DOI: | 10.1163/156856209X427005) |
| Popis: | It was found previously that membranes based on co-polymers of acrylonitrile (AN) and 2-acrylamido-2-methyl-propansulfonic acid (AMPS) greatly stimulated the functionality and survival of primary hepatocytes. In those studies, however, the pure AN-AMPS co-polymer had poor membrane-forming properties, resulting in quite dense rubber-like membranes. Hence, membranes with required permeability and optimal biocompatibility were obtained by blending the AN-AMPS co-polymer with poly(acrylonitrile) homopolymer (PAN). The amount of PAN (P) and AN-AMPS (A) in the blend was varied from pure PAN (P/A-100/0) over P/A-75/25 and P/A-50/50 to pure AN-AMPS co-polymer (P/A-0/100). A gradual decrease of molecular cut-off of membranes with increase of AMPS concentration was found, which allows tailoring membrane permeability as necessary. C3A hepatoblastoma cells were applied as a widely accepted cellular model for assessment of hepatocyte behaviour by attachment, viability, growth and metabolic activity. It was found that the blend P/A-50/50, which possessed an optimal permeability for biohybrid liver systems, supported also the attachment, growth and function of C3A cells in terms of fibronectin synthesis and P-450 isoenzyme activity. Hence, blend membranes based on a one to one mixture of PAN and AN-AMPS combine sufficient permeability with the desired cellular compatibility for application in bioreactors for liver replacement. |
| Databáze: | OpenAIRE |
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