| Autor: |
Bargar, Connor, De Luca, Chiara Maria Giulia, Devigili, Grazia, Elia, Antonio Emanuele, Cilia, Roberto, Portaleone, Sara Maria, Wang, Wen, Tramacere, Irene, Bistaffa, Edoardo, Cazzaniga, Federico Angelo, Felisati, Giovanni, Legname, Giuseppe, Di Fonzo, Alessio, Xu, Rong, Gunzler, Steven Alexander, Giaccone, Giorgio, Eleopra, Roberto, Chen, Shu Guang, Moda, Fabio |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.6084/m9.figshare.17163335.v1 |
| Popis: |
Additional file 1. Detection of ��Syn_RT-QuIC seeding activity triggered by the brain homogenates of patients with PD, MSA-P, and MSA-C. We tested the performance of the new ��Syn_RT-QuIC protocol using the brain homogenates (BH) of autopsy-confirmed cases of PD (n = 1), MSA-P (n = 1), and MSA-C (n = 1) as sources of different ��SynD strains. The BH of a patient not affected by ��-synucleinopathy (NAS) was used as control. Immunohistochemical analysis revealed the presence of distinct ��SynD aggregates in the brain of tested patients, except for NAS. MSA-P and NAS images were taken using 10x magnification. MSA-C and PD images were taken using 40x magnification (a). Frozen brain samples (substantia nigra for PD, striatum for MSA-P, cerebellum for MSA-C, and frontal cortex for NAS) were homogenized at 10% in PBS (weight/volume) and diluted at 10��� 12. One ��l of each BH was added to 49 ��L of reaction mix and subjected to ��Syn_RT-QuIC analysis. The results indicate that PD (light green line), MSA-P (blue line), and MSA-C (grey line) induced ��Syn_RT-QuIC seeding activity before the time threshold set at 13 h while that of the patient with NAS (purple line) did not (b). Each sample was analyzed in quadruplicate. Curves represented in the graph were obtained by plotting the average fluorescence intensities of each sample against time. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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