Pilot-Scale Polyhydroxyalkanoate Production from Organic Waste: Process Characteristics at High pH and High Ammonium Concentration
| Autor: | Jelmer Tamis, João Sousa, Robbert Kleerebezem, René A. Rozendal, Michel Mulders, Henk Dijkman, Ben Abbas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Environmental Engineering
Municipal solid waste Chemistry 0208 environmental biotechnology Pilot scale 02 engineering and technology Biodegradable waste Pulp and paper industry Polyhydroxyalkanoates 020801 environmental engineering chemistry.chemical_compound Volatile fatty acids Scientific method Environmental Chemistry Ammonium Leachate General Environmental Science Civil and Structural Engineering |
| Zdroj: | Journal of Environmental Engineering, 146(7) |
| ISSN: | 0733-9372 |
| Popis: | Polyhydroxyalkanoate (PHA) accumulating microbial enrichment was established on volatile fatty acids (VFAs) containing leachate derived from the organic fraction of municipal solid waste (OFMSW). The enrichment was based on a 12-h feast-famine batch cycle and an exchange ratio of 50% in which VFAs were completely consumed in less than 50 min during stable periods of operation. No pH control was applied in the system, and the pH went as high as 9 due to the presence of amongst others, ammonia [500 mg·L-1 total ammonia nitrogen (TAN) on average]. The degree of enrichment was evaluated with fluorescence in situ hybridization (FISH), and a yet unknown genus of large (3-5 μm diameter) beta-proteobacteria appeared dominant in the culture. A method for estimating the fraction of PHA accumulating active biomass in the total volatile suspended solids was established, and the results indicated an increase of this fraction from 25% to 56% after implementing two modifications in the operational protocol: (1) a pretreatment of the substrate removing virtually all settleable solids; and (2) a settling phase in the enrichment reactor after the feast phase, selectively removing nonsettleable solids and slowly degradable substrates. The PHA accumulation potential of the culture was 77±18 wt% PHA (n=3) after 3 h in batch accumulation experiments. The results suggest the potential feasibility of PHA production under conditions that were previously considered economically favorable but technically difficult. |
| Databáze: | OpenAIRE |
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