l -Arginine Protects Ovine Intestinal Epithelial Cells from Lipopolysaccharide-Induced Apoptosis through Alleviating Oxidative Stress
Autor: | Shuang Guo, Yin Yu, Along Peng, Hongrong Wang, Mengzhi Wang, Hao Zhang |
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Rok vydání: | 2019 |
Předmět: |
Lipopolysaccharides
0106 biological sciences GPX1 Lipopolysaccharide Arginine Cell Survival SOD2 Apoptosis Protective Agents medicine.disease_cause 01 natural sciences Superoxide dismutase chemistry.chemical_compound medicine Animals Caspase 8 Sheep biology Superoxide Dismutase 010401 analytical chemistry Epithelial Cells General Chemistry Catalase Molecular biology Caspase 9 0104 chemical sciences Intestines Heme oxygenase Oxidative Stress chemistry biology.protein General Agricultural and Biological Sciences Heme Oxygenase-1 Oxidative stress 010606 plant biology & botany |
Zdroj: | Journal of Agricultural and Food Chemistry. 67:1683-1690 |
ISSN: | 1520-5118 0021-8561 |
Popis: | This research aims to explore the effect of l-arginine (Arg) upon lipopolysaccharide (LPS)-induced induction of the oxidative stress as well as subsequent apoptosis within ovine intestinal epithelial cells (IOECs). Through a 16 h incubation, cells were divided into four groups and the medium was replaced with different medium as follows: (1) control (Con), Arg-free Dulbecco's modified Eagle's F12 Ham medium (DMEM); (2) Arg treatment, Arg-free DMEM supplemented with 100 μM Arg; (3) LPS treatment, Arg-free DMEM supplemented with 10 μg/mL LPS; (4) LPS with Arg treatment, Arg-free DMEM supplemented with both 10 μg/mL LPS and 100 μM Arg. After culturing for 24 h in different mediums, some characteristics of cells in the four groups were measured. Addition of Arg increased cell viability induced with LPS compared with the LPS group ( p < 0.05). Arg significantly decreased the release of dehydrogenase (LDH) and the production of malonaldehyde (MDA) ( p < 0.05) within IOECs challenged by the LPS. Compared with the LPS group, cells treated with Arg and Arg + LPS increased ( p < 0.05) mRNA as well as protein expression of glutathione peroxidase 1 (GPx1), catalase (CAT), superoxide dismutase 2 (SOD2), B-cell lymphoma 2 (Bcl2), quinone oxidoreductase 1 (NQO1), heme oxygenase (HO-1), and nuclear factor erythroid 2-related factor 2 (Nrf2). IOEC treatment with Arg reduced significantly ( p < 0.05) apoptosis induced by the LPS (12.58 ± 0.79%). The results showed that Arg promoted the protein expression of Nrf2, up-regulated expression of the phase II metabolizing enzymes (NQO1 and HO-1), as well as antioxidative enzymes (GPx1, CAT, and SOD2) for alleviating oxidative injury and protected IOECs from LPS-induced apoptosis. |
Databáze: | OpenAIRE |
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