Heat stroke induces autophagy as a protection mechanism against neurodegeneration in the brain
| Autor: | Chu Dang Tsai, Chou Hui Hu, Yuh Feng Lin, Tsung Ta Liu, Jia Yi Wang, Chuan Wang Li |
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| Rok vydání: | 2010 |
| Předmět: |
Autophagosome
Heat Stroke Central nervous system Immunoblotting Critical Care and Intensive Care Medicine Rats Sprague-Dawley Cadaverine Phagosomes medicine Autophagy Animals Chemistry Neurodegeneration Brain medicine.disease Immunohistochemistry Cell biology Rats medicine.anatomical_structure Cerebral cortex Nerve Degeneration Emergency Medicine Neuron Nucleus Pyknosis |
| Zdroj: | Shock (Augusta, Ga.). 34(6) |
| ISSN: | 1540-0514 |
| Popis: | Heat stroke (HS) is defined clinically as a condition when core body temperature rises above 40°C and is accompanied by central nervous system abnormalities. In this study, we established a rat model of HS by exposing anesthetized rats to elevated ambient temperature (40°C) until core temperature reaching 40.5°C (HS onset). The rat was immediately removed from heating chamber, allowed recovery for various time periods, and killed for histological and biochemical studies. Our results indicated neuronal shrinkage and pyknosis of the nucleus and sustained up to 12 h recovery time in cerebral cortex. Elevated expression of autophagy-related proteins, including microtubule associated protein light chain 3 and beclin 1 in cortical tissue at various times (3, 6, 12 h) of recovery was observed. In addition, the number of autophagosomes stained by monodansylcadaverine, a specific autophagosome marker, increased after heat exposure but was reduced by pretreatment with 3-methyladenine, an autophagy inhibitor. Furthermore, heat exposure increased neuronal degeneration in cortical tissue, as evidenced by staining with the fluorescent dye Fluoro-Jade B for degenerating neuron. Pretreatment with 3-methyladenine in HS rats aggravated neurodegeneration. Taken together, these results suggest that HS induces autophagy as a protection mechanism against neurodegeneration. Modulation of autophagy may provide a potential therapeutic approach for HS and await further research. |
| Databáze: | OpenAIRE |
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