The Impact of Methylenetetrahydrofolate Reductase (MTHFR) Sperm Methylation and Variants on Semen Parameters and the Chance of Recurrent Pregnancy Loss in the Couple
| Autor: | Bahia-Namavar Jahromi, Shiva Poorang, Najmeh Moein-Vaziri, Zahra Anvar, Mahnaz Banaei, Seyed Mohammad Bagher Tabei, Seyed Alireza Dastgheib, Behrouz Gharesi-Fard, Shokoufeh Abdollahi |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Abortion Habitual Bisulfite sequencing Semen Single-nucleotide polymorphism Biology Methylation Polymorphism Single Nucleotide General Biochemistry Genetics and Molecular Biology Andrology Pregnancy medicine Humans Genetic Predisposition to Disease Epigenetics Allele Methylenetetrahydrofolate Reductase (NADPH2) medicine.disease Sperm Spermatozoa digestive system diseases Semen Analysis Methylenetetrahydrofolate reductase biology.protein Female |
| Zdroj: | Clinical laboratory. 64(7) |
| ISSN: | 1433-6510 |
| Popis: | BACKGROUND Recurrent pregnancy loss (RPL) defined as three or more consecutive spontaneous miscarriages before the 20th week of gestation is caused by different factors including genetic and epigenetic background. However the involvement of paternal background on RPL is an interesting novel argument, which is not well studied. The main focus of the present study was to investigate for the association of paternal methylenetetrahydrofolate reductase (MTHFR) epigenotypes with sperm parameters and RPL. Moreover, the frequency of two of MTHFR Single Nucleotide Polymorphisms (SNPs) in males was assessed. METHODS This is a case-control study. Methylation Specific PCR (MSP) was used to evaluate the methylation status of MTHFR promoter on sperm DNA of 25 male partners of RPL and 25 male partners of non-RPL couples. PCR-RFLP method was used to analyze 1,298 A>C (rs1801131) and 677 C>T (rs1801133) polymorphisms. RESULTS No significant difference was observed in frequency of methylated MTHFR epigenotype between RPL and non-RPL males. Furthermore, methylated MTHFR epigenotype was more frequent (but not statistically significant) among men with abnormal sperm parameters compared to normal-sperm men. Among studied polymorphisms, only the mutated allele of C677T showed statistically higher prevalence among RPL males. CONCLUSIONS Although our results do not establish any connection between MTHFR epigenotypes and RPL they do highlight the impact of C677T in the pathology. |
| Databáze: | OpenAIRE |
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