Moderate intake of n-3 fatty acids is associated with stable erythrocyte resistance to oxidative stress in hypertriglyceridemic subjects
| Autor: | Denis Blache, Claudia Rodriguez, Suzanne Lussier-Cacan, Lucie Boulet, Nancy Doyle, Louis-Jacques Fortin, Alain Piolot, Laurence Mabile, Jean Davignon |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_specialty Erythrocytes medicine.medical_treatment Phospholipid Amidines Medicine (miscellaneous) Biology Hemolysis chemistry.chemical_compound Internal medicine Fatty Acids Omega-3 medicine Humans Unsaturated fatty acid Chromatography High Pressure Liquid chemistry.chemical_classification Hypertriglyceridemia Nutrition and Dietetics Cholesterol Vitamin E Erythrocyte Membrane Fatty acid Middle Aged Fish oil medicine.disease Oxidative Stress Endocrinology chemistry Female |
| Zdroj: | The American journal of clinical nutrition. 74(4) |
| ISSN: | 0002-9165 |
| Popis: | Background The important triacylglycerol-lowering capacity of n-3 fatty acids is counterbalanced by their inherent sensitivity to oxidation. Inconsistent results about the latter have been reported in hypertriglyceridemic individuals. After incorporation into cell membranes, n-3 fatty acids may alter membrane-related functions. In view of the distinct composition of hypertriglyceridemic membranes and the prooxidant status in this condition, it can be surmised that cell enrichment with the oxidizable n-3 fatty acids will be associated with an increased hemolytic process. Objective We sought to evaluate the effect of fish oil consumption on n-3 fatty acid incorporation into erythrocyte membranes and subsequent ex vivo oxidative-stress-induced hemolysis in normotriglyceridemic and hypertriglyceridemic subjects. Design Sixteen normotriglyceridemic and 12 hypertriglyceridemic subjects were given 6 g fish oil/d for 8 wk. Blood samples were collected before and 4 and 8 wk after treatment. Resistance to 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis was assayed in fresh erythrocyte suspensions, and erythrocyte samples were stored at -70 degrees C for later analysis of cholesterol, hemoglobin, fatty acids, vitamin E, and glutathione peroxidase activity. Results Fish oil supplementation induced n-3 fatty acid incorporation in normotriglyceridemic and hypertriglyceridemic erythrocyte membranes without decreasing their resistance to AAPH. n-3 Fatty acids significantly protected normotriglyceridemic but not hypertriglyceridemic erythrocytes against hemolysis. In normotriglyceridemic subjects only, the higher resistance to hemolysis correlated with changes in cell vitamin E. Conclusion Although they exhibit a high susceptibility to oxidation, n-3 fatty acids may preserve membrane integrity and represent an added benefit in the treatment of hypertriglyceridemic patients. |
| Databáze: | OpenAIRE |
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