Impact of Roux-en-Y gastric bypass surgery on rat intestinal glucose transport
Autor: | Anita Balakrishnan, Ali Tavakkolizadeh, Adam T. Stearns |
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Rok vydání: | 2010 |
Předmět: |
Male
medicine.medical_specialty Glycosylation Physiology Gastric Bypass Gene Expression Cell Count Biology medicine.disease_cause Gastroenterology digestive system Intestinal absorption Jejunum Rats Sprague-Dawley Eating Sodium-Glucose Transporter 1 Intestinal mucosa Mucosal Biology Physiology (medical) Diabetes mellitus Internal medicine medicine Animals Intestinal Mucosa Hepatology Gastric bypass surgery Stomach Body Weight Glucose transporter nutritional and metabolic diseases Biological Transport medicine.disease Roux-en-Y anastomosis Surgery Circadian Rhythm Rats Intestines Molecular Weight medicine.anatomical_structure Enterocytes Glucose Phlorhizin Intestinal Absorption Goblet Cells Protein Processing Post-Translational |
Zdroj: | American journal of physiology. Gastrointestinal and liver physiology. 297(5) |
ISSN: | 1522-1547 |
Popis: | Roux-en-Y gastric bypass (RYGB) has become the gold-standard bariatric procedure, partly because of the rapid resolution of accompanying diabetes. There is increasing evidence this is mediated by duodenal exclusion. We hypothesize that duodenal exclusion suppresses intestinal Na+/glucose cotransporter SGLT1-mediated glucose transport, improving glucose handling, and aimed to test this in a rodent RYGB model. Sprague-Dawley rats underwent sham procedure or duodenal exclusion by RYGB (10 cm Roux, 16 cm biliopancreatic limbs). Animals were maintained for 3 wk on a Western diet, before harvest at 10 AM, 4 PM, and 10 PM. Sections were taken from each limb for hematoxylin and eosin staining, and morphological assessment was performed. Functional glucose uptake studies, along with Western blotting and quantitative PCR, were performed on Roux limb. Histology showed morphometric changes in Roux and common limbs, with increase in villus height and crypt depth compared with BP and sham jejunum. Despite this, glucose transport was reduced by up to 68% ( P < 0.001) in the Roux limb compared with sham jejunum. Normal diurnal rhythms in glucose uptake were ablated. This occurred at a posttranscriptional level, with little change in message but appearance of different weight species of Sglt1 on Western blotting. We have shown duodenal exclusion significantly influences both intestinal structure and glucose transport function, with glucose absorptive capacity reduced after RYGB. This provides a novel mechanistic explanation for some of the antidiabetic effects of RYGB. |
Databáze: | OpenAIRE |
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